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Title: | Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white |
Keywords: | Functionalization of polymers Morphology Porous materials Proteins |
Issue Date: | Oct-2022 |
Publisher: | Canadian Society for Chemical Engineering (CSChE) |
Citation: | MEIRA, A. C. F. de O. et al. Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white. The Canadian Journal of Chemical Engineering, [S.l.], p. 1-15, Oct. 2022. DOI: 10.1002/cjce.24703. |
Abstract: | The application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with l-phenylalanine (cryogel-Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier-transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mgphenylalanine gcryogel−1. Cryogel-Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200°C. Differential scanning calorimetry and X-ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 × 10−13 m2, axial dispersion ranging from 10−7 to 10−6 m2 s−1, and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g−1) was obtained using sodium thiocyanate saline solution (0.025 mol L−1, pH 5.0). The ability of the cryogel-Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml−1), purity (87.92%), purification factor (11.49), and sulphate-polyacrylamide electrophoresis gel (SDS-PAGE) electrophoresis gel. |
URI: | https://onlinelibrary.wiley.com/doi/10.1002/cjce.24703 http://repositorio.ufla.br/jspui/handle/1/56514 |
Appears in Collections: | DCA - Artigos publicados em periódicos |
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