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Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Meira, Ana Cristina Freitas de Oliveira | - |
dc.creator | Silva, Richard Marins da | - |
dc.creator | Neves, Isabelle Cristina Oliveira | - |
dc.creator | Minim, Luis Antônio | - |
dc.creator | Veríssimo, Lizzy Ayra Alcântara | - |
dc.creator | Resende, Jaime Vilela de | - |
dc.date.accessioned | 2023-04-05T18:21:50Z | - |
dc.date.available | 2023-04-05T18:21:50Z | - |
dc.date.issued | 2022-10 | - |
dc.identifier.citation | MEIRA, A. C. F. de O. et al. Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white. The Canadian Journal of Chemical Engineering, [S.l.], p. 1-15, Oct. 2022. DOI: 10.1002/cjce.24703. | pt_BR |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/10.1002/cjce.24703 | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/56514 | - |
dc.description.abstract | The application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with l-phenylalanine (cryogel-Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier-transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mgphenylalanine gcryogel−1. Cryogel-Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200°C. Differential scanning calorimetry and X-ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 × 10−13 m2, axial dispersion ranging from 10−7 to 10−6 m2 s−1, and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g−1) was obtained using sodium thiocyanate saline solution (0.025 mol L−1, pH 5.0). The ability of the cryogel-Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml−1), purity (87.92%), purification factor (11.49), and sulphate-polyacrylamide electrophoresis gel (SDS-PAGE) electrophoresis gel. | pt_BR |
dc.language | en_US | pt_BR |
dc.publisher | Canadian Society for Chemical Engineering (CSChE) | pt_BR |
dc.rights | restrictAccess | pt_BR |
dc.source | The Canadian Journal of Chemical Engineering (CJCE) | pt_BR |
dc.subject | Functionalization of polymers | pt_BR |
dc.subject | Morphology | pt_BR |
dc.subject | Porous materials | pt_BR |
dc.subject | Proteins | pt_BR |
dc.title | Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DCA - Artigos publicados em periódicos |
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