Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/56514
Registro completo de metadados
Campo DCValorIdioma
dc.creatorMeira, Ana Cristina Freitas de Oliveira-
dc.creatorSilva, Richard Marins da-
dc.creatorNeves, Isabelle Cristina Oliveira-
dc.creatorMinim, Luis Antônio-
dc.creatorVeríssimo, Lizzy Ayra Alcântara-
dc.creatorResende, Jaime Vilela de-
dc.date.accessioned2023-04-05T18:21:50Z-
dc.date.available2023-04-05T18:21:50Z-
dc.date.issued2022-10-
dc.identifier.citationMEIRA, A. C. F. de O. et al. Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white. The Canadian Journal of Chemical Engineering, [S.l.], p. 1-15, Oct. 2022. DOI: 10.1002/cjce.24703.pt_BR
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1002/cjce.24703pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/56514-
dc.description.abstractThe application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with l-phenylalanine (cryogel-Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier-transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mgphenylalanine gcryogel−1. Cryogel-Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200°C. Differential scanning calorimetry and X-ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 × 10−13 m2, axial dispersion ranging from 10−7 to 10−6 m2 s−1, and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g−1) was obtained using sodium thiocyanate saline solution (0.025 mol L−1, pH 5.0). The ability of the cryogel-Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml−1), purity (87.92%), purification factor (11.49), and sulphate-polyacrylamide electrophoresis gel (SDS-PAGE) electrophoresis gel.pt_BR
dc.languageen_USpt_BR
dc.publisherCanadian Society for Chemical Engineering (CSChE)pt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceThe Canadian Journal of Chemical Engineering (CJCE)pt_BR
dc.subjectFunctionalization of polymerspt_BR
dc.subjectMorphologypt_BR
dc.subjectPorous materialspt_BR
dc.subjectProteinspt_BR
dc.titlePerformance of pseudo-specific cryogel in lysozyme purification from chicken egg whitept_BR
dc.typeArtigopt_BR
Aparece nas coleções:DCA - Artigos publicados em periódicos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.