Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/50955
Título: Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil
Palavras-chave: Housekeeping genes
Symbiotic genes
Authentication
Symbiotic efficiency
Leguminosas nodulantes
Genes simbióticos
Eficiência simbiótica
Data do documento: 2022
Editor: Escola Superior de Agricultura "Luiz de Queiroz" - USP/ESALQ
Citação: LOPEZ, B. D. O. et al. Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil. Scientia Agricola, Piracicaba, v. 79, n. 2, e20200238, 2022. DOI: http://doi.org/10.1590/1678-992X-2020-0238.
Resumo: Identification and selection of nitrogen-fixing bacterial strains for inoculation into native leguminous tree species can assist in the recovery of degraded areas. Additionally, native strains from these areas are genetic resources adapted to these conditions and are thus suitable for selection. The aim of this study was to symbiotically and genetically characterize 18 bacterial strains from the Rhizobium and Bradyrhizobium genera isolated from Machaerium nyctitans, Platypodium elegans, and Ormosia arborea grown in a nursery in an iron mining area. Three experiments were conducted under axenic conditions in a greenhouse. The nodulation capacity of the strains was evaluated by the number (NN) and dry matter (NDM) of nodules. Symbiotic efficiency was evaluated based on the following parameters: SPAD index (SPAD), shoot dry matter (SDM), root dry matter (RDM), and total dry matter (TDM) of the plants, relative efficiency (RE), shoot nitrogen content (SNC), and total nitrogen content in the plant (TNC). The atpD and gyrB housekeeping genes and the nifH gene were sequenced for phylogenetic analysis, and the nodC and nodD symbiotic genes of the strains were amplified. Out of the 18 strains, 16 were authenticated by nodulation capacity in the species of origin. The SPAD variable allowed for the detection of differences between treatments before the SDM. Additionally, the SPAD index showed correlation with TNC, and the strain Bradyrhizobium sp., UFLA01-839, which may represent a new species, was outstanding in Machaerium nyctitans. The nifH, nodD, and nodC genes were detected in UFLA01-839.
URI: http://repositorio.ufla.br/jspui/handle/1/50955
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