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Campo DC | Valor | Idioma |
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dc.creator | Lopez, Bruna Daniela Ortiz | - |
dc.creator | Teixeira, Anita Fernanda dos Santos | - |
dc.creator | Michel, Daniele Cabral | - |
dc.creator | Guimarães, Amanda Azarias | - |
dc.creator | Costa, Amanda Monique da | - |
dc.creator | Costa, Juliana Santos | - |
dc.creator | Pereira, Marcela de Souza | - |
dc.creator | Duarte, Bárbara Luyse Marques | - |
dc.creator | Moreira, Fatima Maria de Souza | - |
dc.date.accessioned | 2022-08-12T22:03:23Z | - |
dc.date.available | 2022-08-12T22:03:23Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | LOPEZ, B. D. O. et al. Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil. Scientia Agricola, Piracicaba, v. 79, n. 2, e20200238, 2022. DOI: http://doi.org/10.1590/1678-992X-2020-0238. | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/50955 | - |
dc.description.abstract | Identification and selection of nitrogen-fixing bacterial strains for inoculation into native leguminous tree species can assist in the recovery of degraded areas. Additionally, native strains from these areas are genetic resources adapted to these conditions and are thus suitable for selection. The aim of this study was to symbiotically and genetically characterize 18 bacterial strains from the Rhizobium and Bradyrhizobium genera isolated from Machaerium nyctitans, Platypodium elegans, and Ormosia arborea grown in a nursery in an iron mining area. Three experiments were conducted under axenic conditions in a greenhouse. The nodulation capacity of the strains was evaluated by the number (NN) and dry matter (NDM) of nodules. Symbiotic efficiency was evaluated based on the following parameters: SPAD index (SPAD), shoot dry matter (SDM), root dry matter (RDM), and total dry matter (TDM) of the plants, relative efficiency (RE), shoot nitrogen content (SNC), and total nitrogen content in the plant (TNC). The atpD and gyrB housekeeping genes and the nifH gene were sequenced for phylogenetic analysis, and the nodC and nodD symbiotic genes of the strains were amplified. Out of the 18 strains, 16 were authenticated by nodulation capacity in the species of origin. The SPAD variable allowed for the detection of differences between treatments before the SDM. Additionally, the SPAD index showed correlation with TNC, and the strain Bradyrhizobium sp., UFLA01-839, which may represent a new species, was outstanding in Machaerium nyctitans. The nifH, nodD, and nodC genes were detected in UFLA01-839. | pt_BR |
dc.language | en | pt_BR |
dc.publisher | Escola Superior de Agricultura "Luiz de Queiroz" - USP/ESALQ | pt_BR |
dc.rights | Attribution 4.0 International | * |
dc.rights | acesso aberto | pt_BR |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.source | Scientia Agricola | pt_BR |
dc.subject | Housekeeping genes | pt_BR |
dc.subject | Symbiotic genes | pt_BR |
dc.subject | Authentication | pt_BR |
dc.subject | Symbiotic efficiency | pt_BR |
dc.subject | Leguminosas nodulantes | pt_BR |
dc.subject | Genes simbióticos | pt_BR |
dc.subject | Eficiência simbiótica | pt_BR |
dc.title | Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DCS - Artigos publicados em periódicos |
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ARTIGO_Genetic and symbiotic characterization of rhizobia nodulating legumes in a mining area in southeast Brazil.pdf | 2,04 MB | Adobe PDF | Visualizar/Abrir |
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