Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/39929
Title: Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice
Keywords: Brucella abortus
Rough strain
Live vaccine
Issue Date: Aug-2010
Publisher: Elsevier
Citation: LACERDA, T. L. S. et al. Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice. Vaccine, [S.l.], v. 28, n. 34, p. 5627-5634, Aug. 2010. DOI: 10.1016/j.vaccine.2010.06.023.
Abstract: Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusΔwbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-α when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. ΔwbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.
URI: https://www.sciencedirect.com/science/article/pii/S0264410X10008236
http://repositorio.ufla.br/jspui/handle/1/39929
Appears in Collections:DBI - Artigos publicados em periódicos

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