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DC Field | Value | Language |
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dc.creator | Lacerda, Thaís Lourdes Santos | - |
dc.creator | Cardoso, Patrícia Gomes | - |
dc.creator | Almeida, LeonardoAugusto de | - |
dc.creator | Camargo, Ilana Lopes Baratella da Cunha | - |
dc.creator | Afonso, Daniela Almeida Freitas | - |
dc.creator | Trant, Cyntia Cardoso | - |
dc.creator | Macedo, Gilson Costa | - |
dc.creator | Campos, Eleonora | - |
dc.creator | Cravero, Silvio L. | - |
dc.creator | Salcedo, Suzana P. | - |
dc.creator | Gorvel, Jean-Pierre | - |
dc.creator | Oliveira, Sérgio Costa | - |
dc.date.accessioned | 2020-04-09T20:33:49Z | - |
dc.date.available | 2020-04-09T20:33:49Z | - |
dc.date.issued | 2010-08 | - |
dc.identifier.citation | LACERDA, T. L. S. et al. Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice. Vaccine, [S.l.], v. 28, n. 34, p. 5627-5634, Aug. 2010. DOI: 10.1016/j.vaccine.2010.06.023. | pt_BR |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0264410X10008236 | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/39929 | - |
dc.description.abstract | Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusΔwbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-α when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. ΔwbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence. | pt_BR |
dc.language | en_US | pt_BR |
dc.publisher | Elsevier | pt_BR |
dc.rights | restrictAccess | pt_BR |
dc.source | Vaccine | pt_BR |
dc.subject | Brucella abortus | pt_BR |
dc.subject | Rough strain | pt_BR |
dc.subject | Live vaccine | pt_BR |
dc.title | Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice | pt_BR |
dc.type | Artigo | pt_BR |
Appears in Collections: | DBI - Artigos publicados em periódicos |
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