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http://repositorio.ufla.br/jspui/handle/1/57882
Title: | Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD |
Keywords: | Clinical samples Sensitivity White spot syndrome virus (WSSV) Real-time PCR White spot disease (WSD) |
Issue Date: | 2013 |
Publisher: | Sociedade Brasileira de Microbiologia |
Citation: | LEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013. |
Abstract: | The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. |
URI: | http://repositorio.ufla.br/jspui/handle/1/57882 |
Appears in Collections: | DMV - Artigos publicados em periódicos |
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