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Title: | Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods |
Other Titles: | Detecção de Campylobacter spp. em carcaças de frango de corte resfriadas e congeladas pelos métodos imunoenzimático, PCR e PCR em tempo real |
Keywords: | Reação em cadeia da polimerase Campylobacter spp. - Métodos de detecção C. jejuni - Contaminação Polymerase Chain Reaction Campylobacter spp. - Detection methods C. jejuni - Contamination |
Issue Date: | 2018 |
Publisher: | Universidade Federal de Santa Maria |
Citation: | REIS, L. P. et al. Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods. Ciência Rural, Santa Maria, v. 48, n. 2, p. 1-7, 2018. |
Abstract: | In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni. |
URI: | http://repositorio.ufla.br/jspui/handle/1/57666 |
Appears in Collections: | DMV - Artigos publicados em periódicos |
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