Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/57666
Title: Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods
Other Titles: Detecção de Campylobacter spp. em carcaças de frango de corte resfriadas e congeladas pelos métodos imunoenzimático, PCR e PCR em tempo real
Keywords: Reação em cadeia da polimerase
Campylobacter spp. - Métodos de detecção
C. jejuni - Contaminação
Polymerase Chain Reaction
Campylobacter spp. - Detection methods
C. jejuni - Contamination
Issue Date: 2018
Publisher: Universidade Federal de Santa Maria
Citation: REIS, L. P. et al. Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods. Ciência Rural, Santa Maria, v. 48, n. 2, p. 1-7, 2018.
Abstract: In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.
URI: http://repositorio.ufla.br/jspui/handle/1/57666
Appears in Collections:DMV - Artigos publicados em periódicos

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