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Title: | Endo-β-Mananase e α-galactosidase e suas relações com a tolerância à termoinibição em sementes de alface |
Other Titles: | Endo-β-Mananase and α-galactosidase and their relationships to the tolerance to thermoinhibition in lettuce seeds |
Authors: | Santos, Heloisa Oliveira dos Gomes, Luiz Antônio Augusto Santos, Heloisa Oliveira dos Gomes, Luiz Antônio Augusto Oliveira, Cleiton Lourenço de Mann, Renata Silva Dias, Denise Cunha Fernandes dos Santos Gomes, Luiz Antônio Augusto |
Keywords: | Alface - Sementes Sementes - Termoinibição Atividade enzimática Alface - Melhoramento genético Termotolerância Altas temperaturas Lettuce - Seeds Seeds - Thermoinhibition Enzymatic activity Lettuce - Genetic improvement Thermotolerance High temperatures |
Issue Date: | 2-Aug-2022 |
Publisher: | Universidade Federal de Lavras |
Citation: | CAVASIN, P. Y. Endo-β-Mananase e α-galactosidase e suas relações com a tolerância à termoinibição em sementes de alface. 2022. 49 p. Tese (Doutorado em Agronomia/Fitotecnia) – Universidade Federal de Lavras, Lavras, 2022. |
Abstract: | Temperature strongly influences the germination of lettuce seeds (Lactuca sativa L.) due to the thermoinhibition effect. The aim of this study was to determine the enzymatic activity and gene expression of different enzymes associated with the thermoinhibition effect of lettuce seeds. Seeds of the cultivars Everglades (tolerant to thermoinhibition) and Verônica (sensitive to thermoinhibition), the reciprocal hybrids, originated from the cross between the two cultivars and the F2 generation were used. The seeds were submitted to germination tests, first germination count, germination speed index (VIG) and enzymatic tests (Catalase, superoxide dismutase, ascorbate peroxidase and endo-β-mannanase). Subsequently, the expression of genes related to endo-β-mannanase and α-galactosidase enzymes was evaluated by RTq-PCR. Regarding the physiological tests, the Everglades cultivar, as well as its hybrids, were less thermoinhibited than the other treatments. The activities of the enzymes superoxide dismutase and endo-β-mannanase showed a direct and more expressive relationship with the alterations in the physiological parameters of thermotolerance. Regarding gene expression, there was variation in transcripts that express endo-β-mannanase and α-galactosidase between the treatments evaluated. However, endo-β-mannanase followed the trend of the thermoinhibition trait and may be associated with this trait in Lactuca sativa seeds. Therefore, it is concluded that endo-β-mannanase is a promising enzymatic and genetic marker associated with thermotolerance and is an important tool in the selection of cultivars in lettuce breeding programs. |
URI: | http://repositorio.ufla.br/jspui/handle/1/50790 |
Appears in Collections: | Agronomia/Fitotecnia - Doutorado (Teses) |
Files in This Item:
File | Description | Size | Format | |
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TESE_Endo-β-Mananase e α-galactosidase e suas relações com a tolerância à termoinibição em sementes de alface.pdf | 939,09 kB | Adobe PDF | View/Open |
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