Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/50790
Title: Endo-β-Mananase e α-galactosidase e suas relações com a tolerância à termoinibição em sementes de alface
Other Titles: Endo-β-Mananase and α-galactosidase and their relationships to the tolerance to thermoinhibition in lettuce seeds
Authors: Santos, Heloisa Oliveira dos
Gomes, Luiz Antônio Augusto
Santos, Heloisa Oliveira dos
Gomes, Luiz Antônio Augusto
Oliveira, Cleiton Lourenço de
Mann, Renata Silva
Dias, Denise Cunha Fernandes dos Santos
Gomes, Luiz Antônio Augusto
Keywords: Alface - Sementes
Sementes - Termoinibição
Atividade enzimática
Alface - Melhoramento genético
Termotolerância
Altas temperaturas
Lettuce - Seeds
Seeds - Thermoinhibition
Enzymatic activity
Lettuce - Genetic improvement
Thermotolerance
High temperatures
Issue Date: 2-Aug-2022
Publisher: Universidade Federal de Lavras
Citation: CAVASIN, P. Y. Endo-β-Mananase e α-galactosidase e suas relações com a tolerância à termoinibição em sementes de alface. 2022. 49 p. Tese (Doutorado em Agronomia/Fitotecnia) – Universidade Federal de Lavras, Lavras, 2022.
Abstract: Temperature strongly influences the germination of lettuce seeds (Lactuca sativa L.) due to the thermoinhibition effect. The aim of this study was to determine the enzymatic activity and gene expression of different enzymes associated with the thermoinhibition effect of lettuce seeds. Seeds of the cultivars Everglades (tolerant to thermoinhibition) and Verônica (sensitive to thermoinhibition), the reciprocal hybrids, originated from the cross between the two cultivars and the F2 generation were used. The seeds were submitted to germination tests, first germination count, germination speed index (VIG) and enzymatic tests (Catalase, superoxide dismutase, ascorbate peroxidase and endo-β-mannanase). Subsequently, the expression of genes related to endo-β-mannanase and α-galactosidase enzymes was evaluated by RTq-PCR. Regarding the physiological tests, the Everglades cultivar, as well as its hybrids, were less thermoinhibited than the other treatments. The activities of the enzymes superoxide dismutase and endo-β-mannanase showed a direct and more expressive relationship with the alterations in the physiological parameters of thermotolerance. Regarding gene expression, there was variation in transcripts that express endo-β-mannanase and α-galactosidase between the treatments evaluated. However, endo-β-mannanase followed the trend of the thermoinhibition trait and may be associated with this trait in Lactuca sativa seeds. Therefore, it is concluded that endo-β-mannanase is a promising enzymatic and genetic marker associated with thermotolerance and is an important tool in the selection of cultivars in lettuce breeding programs.
URI: http://repositorio.ufla.br/jspui/handle/1/50790
Appears in Collections:Agronomia/Fitotecnia - Doutorado (Teses)



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