Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/50097
Título: Insuflação uterina com ozônio em éguas com endometrite induzida por lipopolissacarídeo de Escherichia Coli
Título(s) alternativo(s): Uterine inflation with ozone in mares with Escherichia Coli lipopolysaccharide-induced endometritis
Autores: Souza, José Camisão de
Souza, José Camisão de
Murgas, Luis David Solis
Maculan, Renata
Palavras-chave: Ozonioterapia
Equinos - Endometrite
Equinos - Reprodução
Insuflação uterina
Ozone therapy
Equines - Endometritis
Horses - Reproduction
Uterine insufflation
Data do documento: 2-Mai-2022
Editor: Universidade Federal de Lavras
Citação: ALVES, N. Insuflação uterina com ozônio em éguas com endometrite induzida por lipopolissacarídeo de Escherichia Coli. 2022. 58 p. Dissertação (Mestrado em Ciências Veterinárias) – Universidade Federal de Lavras, Lavras, 2022.
Resumo: This study aimed to evaluate whether applications of uterine insufflation with ozone in mares induced to endometritis applying lipopolysaccharide (LPS) from Escherichia coli (E. coli) can reduce the number of inflammatory cells in the uterus after treatment. Twenty mares were submitted to cytological tests, biopsy, culture, and blood count, randomly allocated in three treatments: Ozone Treatment1 (TO1), which consisted of applying the gas directly to the uterus, through insufflation for 6 minutes, at a concentration of 35 mg /mL, 0.25 L/mm of flow and 1.5 liters of gas for each animal; Ozone Treatment2 (TO2), which consisted of the same procedure as treatment 1, however, performed on two consecutive days; and Treatment 3 Negative control (TCN), in which the animals did not receive LPS, but only one application of ozone, as described in treatment 1. Two evaluations were performed, the first comparing the treatments and the second comparing the cytology within each of the treatments. treatments. In the first analysis (between treatments) the first 4 cytologies had similar values, however in cytology 5 the values of polymorphonuclear cells (PMNs) were different (P=0.01), being TO1 (0.21±2.7) and TO2 (10.6±2.9). When the analyzes were comparing the cytology performed in each treatment, the PMN values were different in treatment 1 (P = 0.01), in treatment 2 (P<0.001) and in TCN (P=0.002). Biopsy and culture samples were collected on D0 and D4, the histopathological analyzes were not different between the groups evaluated, therefore, the procedures performed did not cause any apparent damage to the endometrium. In relation to culture, TO1, 12.5% of the animals that had bacteria in culture 1 became negative in culture 2, in TO2 28.6% the results suggest that only one application of O3 was necessary to reduce the amount of PMNs in the cytological samples, being beneficial for the treatment of endometritis, and that two applications were more efficient in terms of reducing the amount of microorganisms in the culture.
URI: http://repositorio.ufla.br/jspui/handle/1/50097
Aparece nas coleções:Ciências Veterinárias - Mestrado (Dissertações)



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