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Title: | Prevalência do operon sfrAAABCD (surfactina) em Bacillus subtilis: uma análise pangenômica |
Other Titles: | Prevalence of the sfrAABCD operon (Surfactin) in Bacillus subtilis: a pangenomic analysis |
Authors: | Pylro, Victor Satler Batista, Cristina Ferreira Silva e Morais, Daniel Kumazawa Melo, Dirceu de Sousa Roesch, Luiz Fernando Wurdig |
Keywords: | Genômica comparativa Montagem híbrida Surfactina GridION Comparative genomics Hybrid assembly Surfactin |
Issue Date: | 4-Oct-2021 |
Publisher: | Universidade Federal de Lavras |
Citation: | FERREIRA, J. K. de L. Prevalência do operon sfrAAABCD (surfactina) em Bacillus subtilis: uma análise pangenômica. 2021. 65 p. Dissertação (Mestrado em Microbiologia Agrícola) – Universidade Federal de Lavras, Lavras, 2021. |
Abstract: | Surfactin is a lipopeptide biosurfactant commonly produced by Bacillus genus bacteria. However, its expression is dependent on several genetic factors of adaptation and communication. Gene prevalence studies are important to help the understanding of these interaction factors. This work aimed to evaluate the surfactin operon (sfrAABCD)’s prevalence in Bacillus subtilis strains, as well as the pangenomic repertoire of the species through comparative genomics. The complete genome of B. subtilis RI4914 was sequenced using a hybrid approach, with the PGM IonTorrent and the GridIONTM platforms. A total of 153 complete genomes classified as B. subtilis were retrieved from the NCBI for comparative study, from which the core genes were used for pangenome and MLST analyses. The genome obtained showed high quality and good accuracy, with completeness of 98.84% and contamination of 1.10%. The comparative genomics analysis showed the open pangenomic profile in B. subtilis and surfactin prevalence in all analyzed genomes, classifying it as a distinctive character of this species. |
URI: | http://repositorio.ufla.br/jspui/handle/1/48311 |
Appears in Collections: | Microbiologia Agrícola - Mestrado (Dissertações) |
Files in This Item:
File | Description | Size | Format | |
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DISSERTAÇÃO_Prevalência do operon sfrAAABCD (surfactina) em Bacillus subtilis: uma análise pangenômica.pdf | 1,64 MB | Adobe PDF | View/Open |
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