Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/48311
Title: Prevalência do operon sfrAAABCD (surfactina) em Bacillus subtilis: uma análise pangenômica
Other Titles: Prevalence of the sfrAABCD operon (Surfactin) in Bacillus subtilis: a pangenomic analysis
Authors: Pylro, Victor Satler
Batista, Cristina Ferreira Silva e
Morais, Daniel Kumazawa
Melo, Dirceu de Sousa
Roesch, Luiz Fernando Wurdig
Keywords: Genômica comparativa
Montagem híbrida
Surfactina
GridION
Comparative genomics
Hybrid assembly
Surfactin
Issue Date: 4-Oct-2021
Publisher: Universidade Federal de Lavras
Citation: FERREIRA, J. K. de L. Prevalência do operon sfrAAABCD (surfactina) em Bacillus subtilis: uma análise pangenômica. 2021. 65 p. Dissertação (Mestrado em Microbiologia Agrícola) – Universidade Federal de Lavras, Lavras, 2021.
Abstract: Surfactin is a lipopeptide biosurfactant commonly produced by Bacillus genus bacteria. However, its expression is dependent on several genetic factors of adaptation and communication. Gene prevalence studies are important to help the understanding of these interaction factors. This work aimed to evaluate the surfactin operon (sfrAABCD)’s prevalence in Bacillus subtilis strains, as well as the pangenomic repertoire of the species through comparative genomics. The complete genome of B. subtilis RI4914 was sequenced using a hybrid approach, with the PGM IonTorrent and the GridIONTM platforms. A total of 153 complete genomes classified as B. subtilis were retrieved from the NCBI for comparative study, from which the core genes were used for pangenome and MLST analyses. The genome obtained showed high quality and good accuracy, with completeness of 98.84% and contamination of 1.10%. The comparative genomics analysis showed the open pangenomic profile in B. subtilis and surfactin prevalence in all analyzed genomes, classifying it as a distinctive character of this species.
URI: http://repositorio.ufla.br/jspui/handle/1/48311
Appears in Collections:Microbiologia Agrícola - Mestrado (Dissertações)



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