Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/41556
Title: In vitro anticoagulant activity of mikania laevigata: deepening the study of the possible interaction between guaco and anticoagulants
Keywords: Herbs
Mikania
Thrombin generation
Warfarin
Ervas
Asteraceae
Anticoagulantes
Varfarina
Issue Date: Dec-2019
Publisher: Wolters Kluwer
Citation: LEITE, P. M. et al. In Vitro Anticoagulant Activity of Mikania laevigata: Deepening the Study of the Possible Interaction Between Guaco and Anticoagulants. Journal of Cardiovascular Pharmacology, [S.I.], v. 74, n. 6, p. 574-583, Dec. 2019.
Abstract: Mikania laevigata, popularly known in Brazil as guaco, is widely used for respiratory disorders. As this plant is rich in coumarins, there is evidence of indications that it may cause bleeding and therefore should not be used concomitantly with anticoagulants. The basis of this information is very theoretical, with no clinical evidence of such contraindication. Thus, the aim of this study was to evaluate the in vitro effect of M. laevigata extract on blood coagulation through prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests, fibrinogen plasma concentration, and the new thrombin generation test, which investigate, with high sensibility, hemostatic changes (CAAE 60904316.6.0000.5149), besides evaluating its qualitative micromolecular composition, providing scientific evidence to support the management of patients taking warfarin. Ethanolic extracts of guaco leaves were incubated with a plasma pool of healthy individuals at concentrations of 1.67, 2.26, and 2.86 mg/mL. The presence of flavonoids, tannins, coumarins, and triterpenes was demonstrated by selective reagents in thin layer chromatography. Benzoylgrandifloric acid, cinnamoylgrandifloric acid, o-coumaric acid, coumarin, and quercetin-3-β-glucoside were identified by coinjection in ultraperformance liquid chromatography. The extract at all concentrations prolonged TP and aPPT and reduced the potential for endogenous thrombin potential by the thrombin generation test. The control plasma had endogenous thrombin potential = 1465 nM/min, and after the addition of M. laevigata extract (2.26 mg/mL), this value was reduced to 1087 nM/min, indicating a lower generation of thrombin. Related to fibrinogen plasma concentration, concentrations of 2.26 and 2.86 mg/mL were effective in reducing plasma fibrinogen levels. These results allow us to conclude that the guaco extract demonstrated an anticoagulant effect in vitro, possibly interfering with intrinsic, extrinsic, and common coagulation pathways. A discussion on the contribution of the identified substances to the activity is also present.
URI: https://journals.lww.com/cardiovascularpharm/Citation/2019/12000/In_Vitro_Anticoagulant_Activity_of_Mikania.14.aspx
http://repositorio.ufla.br/jspui/handle/1/41556
Appears in Collections:DAG - Artigos publicados em periódicos

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