Please use this identifier to cite or link to this item:
http://repositorio.ufla.br/jspui/handle/1/41433
Title: | Phenolic profiling and in vitro bioactivity of Moringa oleifera leaves as affected by different extraction solvents |
Keywords: | Moringa Food metabolomics Enzymatic activity Antioxidant activity Antimicrobial activity Acácia-branca Metabolômica alimentar Atividade enzimática Atividade antioxidante Atividade antimicrobiana |
Issue Date: | Jan-2020 |
Publisher: | Elsevier B.V. |
Citation: | ROCCHETTI, G. et al. Phenolic profiling and in vitro bioactivity of Moringa oleifera leaves as affected by different extraction solvents. Food Research International, [S. I.], v. 127, Jan. 2020. Paginação irregular. |
Abstract: | In this work the (poly)-phenolic profile of Moringa oleifera leaves was comprehensively investigated through untargeted metabolomics, following a homogenizer-assisted extraction (HAE) using three solvent systems, i.e. methanol (HAE-1), methanol-water 50:50 v/v (HAE-2) and ethyl acetate (HAE-3). This approach allowed to putatively annotate 291 compounds, recording mainly flavonoids and phenolic acids. Thereafter, antioxidant capacity, antimicrobial activity and enzyme inhibition were assayed in the different extracts. HAE-1 extract showed the highest total phenolic content (31.84 mg/g), followed by HAE-2 (26.95 mg/g) and HAE-3 (14.71 mg/g). In addition, HAE-1 and HAE-2 extracts exhibited an expressive activity against Bacillus cereus and Listeria innocua. The HAE-2 leaf extract was characterized by the highest DPPH and ABTS values (being 49.55 and 45.26 mgTE/g), while ferric reducing antioxidant power was found to be higher in HAE-1 (58.26 mgTE/g). Finally, the enzyme inhibitory effects of M. oleifera leaf extracts were investigated against five enzymes, namely acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase. All of the tested extracts exhibited inhibitory effects on AChE and BChE with a higher activity for HAE-3 and HAE-1, whilst HAE-1 showed the higher impact on tyrosinase, glucosidase and amylase activities. Taken together, these findings suggest that M. oleifera leaf extracts are a good source of bioactive polyphenols with a potential use in food and pharma industries. |
URI: | https://www.sciencedirect.com/science/article/pii/S0963996919305988#! http://repositorio.ufla.br/jspui/handle/1/41433 |
Appears in Collections: | DCA - Artigos publicados em periódicos |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.