Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/40087
Title: Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography
Keywords: Cryogels - Functionalization
Preparative chromatography
Lysozyme adsorption
Hydrophobic affinity
Criogéis - Funcionalização
Cromatografia preparativa
Adsorção de lisozima
Afinidade hidrofóbica
Issue Date: 2019
Publisher: Taylor & Francis
Citation: OLIVEIRA, A. C. F. de et al. Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography. Separation Science and Technology, [S. l.], 2019. DOI: https://doi.org/10.1080/01496395.2019.1617743.
Abstract: Different protocols for functionalization of cryogels with L-tryptophan were tested for the lysozyme adsorption. The effect of ionic strength and pH on the adsorptive capacity (q) was investigated. The morphological, thermal, and mechanical properties of the cryogels were determined. A maximum adsorption for lysozyme (58.030 mg g–1) was achieved when 5% (v/v) of glutaraldehyde (at pH 6.0) and no methanol were used during the cryogel functionalization. The hydrophobic cryogel presented a porosity of 0.666. A strong single band at 14.3 kDa was visualized in SDS-PAGE, indicating that the hydrophobic affinity cryogels was efficient for the lysozyme capture.
URI: https://www.tandfonline.com/doi/abs/10.1080/01496395.2019.1617743?journalCode=lsst20
http://repositorio.ufla.br/jspui/handle/1/40087
Appears in Collections:DCA - Artigos publicados em periódicos

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