Please use this identifier to cite or link to this item:
http://repositorio.ufla.br/jspui/handle/1/39935
Title: | Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation |
Other Titles: | Otimização das condições de cultivo para produção de tanase por Aspergillus sp. GM4 em fermentação em estado sólido |
Keywords: | Surface response methodology Enzyme Jamun Agricultural residues Metodologia de superfície de resposta Enzima Jambolão Resíduos agrícolas |
Issue Date: | Mar-2015 |
Publisher: | Editora da Universidade Estadual de Maringá (EDUEM) |
Citation: | SOUZA, P. N. da C. et al. Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation. Acta Scientiarum. Biological Sciences, Maringá, v. 37, n. 1, p. 23-30, Jan./Mar. 2015. DOI: 10.4025/actascibiolsci.v37i1.22731. |
Abstract: | Tannase is an industrially important enzyme produced by a large number of microorganisms. This study analyzed the production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) using different vegetable leaves (mango, jamun and coffee) and agricultural residues (coffee husks, rice husks and wheat bran). Among the substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. A lower incubation time was fixed and supplementation with potassium nitrate and tannic acid were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid at 1.53% (w w-1) and potassium nitrate at 2.71% (w w-1). After the optimization process, tannase production increased 4.65-fold, which showed that the statistical experimental design offers a practicable approach to the implementation of optimization of tannase production. |
URI: | http://repositorio.ufla.br/jspui/handle/1/39935 |
Appears in Collections: | DBI - Artigos publicados em periódicos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ARTIGO_Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation.pdf | 867,67 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License