Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/39010
Title: Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
Other Titles: Notas do histograma contribuem para a confiabilidade das estimativas do conteúdo de DNA de Brachiaria spp
Keywords: Brachiaria ruziziensis
Brachiaria brizantha
Colchicine
Conteúdo de DNA nuclear
Colchicina
Issue Date: 2012
Publisher: Universidade Federal de Lavras
Citation: TIMBÓ, A. L. de O.; PEREIRA, R. C.; SANTOS, V. B. dos; SOBRINHO, F. S.; DAVIDE, L. C. Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp. Ciência e Agrotecnologia, Lavras, v. 36, n. 6, p. 599-607, nov./dez. 2012.
Abstract: Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5), coefficient of variation and estimation of DNA content. The best combination for the analysis of Brachiaria spp. DNA content was the use of MgSO4 buffer with R. sativus as a internal reference standard. Genome sizes expressed in picograms of DNA are presented for all genotypes and the importance of the histogram score on the results reliability of DNA content analyses were discussed.
URI: http://repositorio.ufla.br/jspui/handle/1/39010
Appears in Collections:DBI - Artigos publicados em periódicos



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