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Título: | Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
Título(s) alternativo(s): | Perda e restabelecimento da tolerância à dessecação em sementes germinadas de Sesbania virgata (Cav.) (Pers.) |
Palavras-chave: | Abscisic acid Cytological alteration Osmotic stress Ácido abscísico Alteração citológica Estresse osmótico Seeds - Desiccation tolerance Sementes - Tolerância à dessecação |
Data do documento: | Jul-2015 |
Editor: | Universidade Estadual de Maringá |
Citação: | MASETTO, T. E.; FARIA, J. M. FRAIZ, A. C. R. Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.). Acta Scientiarum. Agronomy, Maringá, v. 73, n. 3, p. 313-320, July/Sept. 2015. |
Resumo: | This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length. |
URI: | http://repositorio.ufla.br/jspui/handle/1/38635 |
Aparece nas coleções: | DCF - Artigos publicados em periódicos |
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