Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/37316
Title: Chemical sterilization of culture medium: a low cost alternative to in vitro establishment of plants
Other Titles: Esterilização química do meio de cultura: uma alternativa de baixo custo para o estabelecimento in vitro de plantas
Keywords: Active chlorine
Micropropagation
Contamination
Aseptic cultivation
Eucalyptus benthamii
Cloro ativo
Micropropagação
Contaminação
Cultivo asséptico
Issue Date: Jun-2013
Publisher: Instituto de Pesquisas Florestais
Citation: BRONDANI, G. E. et al. Chemical sterilization of culture medium: a low cost alternative to in vitro establishment of plants. Scientia Forestalis, Piracicaba, v. 41, n. 98, p. 257-264, June 2013.
Abstract: This study aimed at evaluating the in vitro establishment of nodal segments of Eucalyptus benthamii in a non-autoclaved culture medium supplemented with different concentrations of active chlorine (NaClO): P2 - 0.000%, P3 - 0.001%, P4 - 0.003%, P5 - 0.005%, P6 - 0.007% and P7 - 0.010%, v/v. The control consisted of an autoclaved culture medium without active chlorine (P1 - traditional preparation). The percentage of establishment, viable shoots, fungal and bacterial contamination, number of shoots and total shoot length were evaluated after 28 days. The in vitro establishment of nodal segments of E. benthamii was feasible in culture medium supplemented with active chlorine without autoclaving. Concentrations of 0.001% and 0.003% active chlorine produced results most similar to the traditional culture medium. We conclude that treatment with active chlorine is an effective method for tissue culture in Eucalyptus, and has considerable potential to decrease costs and energy expenditure in other plant-culture systems.
URI: https://www.ipef.br/publicacoes/scientia/nr98/cap11.pdf
http://repositorio.ufla.br/jspui/handle/1/37316
Appears in Collections:DCF - Artigos publicados em periódicos

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