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Title: | Haploid identification using tropicalized haploid inducer progenies in maize |
Keywords: | Flow cytometry Zea mays Chromosome counting Chromosome duplication Ploidy level |
Issue Date: | Mar-2018 |
Publisher: | Universidade Federal de Viçosa (UFV), Departamento de Fitotecnia (DFT) |
Citation: | RIBEIRO, C. B. et al. Haploid identification using tropicalized haploid inducer progenies in maize. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 18, n. 1, p. 16-23, Jan./Mar. 2018. DOI: 10.1590/1984-70332018v18n1a3. |
Abstract: | The aim of this study was to identify maize haploid plants and compare the efficiency of identification of maize haploid plants using the R1-nj morphological marker, plant vigor, flow cytometry, chromosome counting, and microsatellite molecular markers under tropical conditions. We also established a protocol for chromosome duplication in maize haploid plants. Fourteen S0:1 and seven S2:3 haploid inducer progenies were crossed with GNZ9501 in 2012/2013 and 2014/2015, respectively. Through use of the R1-nj trait, we were able to identify 552 putative haploid seeds in 2012/2013 and 260 putative haploid seeds in 2014/2015. Only 1.84% were true positives according to flow cytometry in 2012/2013. In 2014/2015, 75% of the putative haploids were true negatives according to molecular markers. Plant vigor had a high proportion of true negatives. Molecular markers and flow cytometry are more efficient in classifying plant ploidy level. Chromosome duplication was efficient in all plants. |
URI: | http://repositorio.ufla.br/jspui/handle/1/33241 |
Appears in Collections: | DBI - Artigos publicados em periódicos |
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File | Description | Size | Format | |
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ARTIGO_Haploid identification using tropicalized haploid inducer progenies in maize.pdf | 690,81 kB | Adobe PDF | View/Open |
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