Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/31824
Título: Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1
Título(s) alternativo(s): Concentração de inóculo e tempo de inoculação para produção de ácido propiônico usando cultura de Lactobacillus helveticus e Propionibacterium freudenreichii
Palavras-chave: Skimmed whey
Lactic bacteria
Propionic fermentation
Organic acids
Soro desnatado
Bactérias láticas
Fermentação propiônica
Ácidos orgânicos
Data do documento: 2017
Editor: Universidade Estadual de Maringá
Citação: NGOME, M. T. et al. Inoculum concentration and inoculation time for propionic acid production from whey using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii PS-1. Acta Scientiarum.Technology, Maringá, v. 39, 2017.
Resumo: The production of propionic acid using skim serum by-products of the dairy industry was investigated, and the influence of inoculum concentration of Propionibacterium freudenreichii PS-1 and its time of inoculation for the production of propionic acid was evaluated using mixed culture of Lactobacillus helveticus and Propionibacterium freudenreichii. Initially, the adjustment of propionic bacteria to higher concentrations of propionic acid was tested and kinetic parameters were determined for growth. A central composite rotational design (CCRD) with 11 experiments was carried out, using different inoculum concentrations of P. freudenreichii, which were added to the cultures of lactic bacteria at different times. The maximum specific growth rate for P. freudenreichii decreased from 0.11 to 0.03 hour-1and the growth time increased from 32h to 100h when concentration of propionic acid in lactate broth increased to 0.25%. The best result for propionic acid production was 3.78 g L-1, inoculating 5.108 CFU mL-1 of propionic bacteria after 6 h. For lactose consumption, the best conditions were inoculum concentration above 5.108 CFU mL-1 and inoculation time of P. freudenreichii after 6 hours. Therefore, it was demonstrated that skimmed whey can be used as a renewable, low-cost raw material for propionic acid production by mixed culture.
URI: http://repositorio.ufla.br/jspui/handle/1/31824
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