Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/31641
Título: Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
Título(s) alternativo(s): Mudanças celulares durante a re-indução da tolerância à dessecação em sementes germinadas de Sesbania virgata (Cav.) Pers.
Palavras-chave: Cell viability
Transmission electron microscopy
Tunel test
Viabilidade celular
Microscopia eletrônica de transmissão
Data do documento: Jul-2016
Editor: Associação Brasileira de Tecnologia de Sementes
Citação: MASETTO, T. E.; FRAIZ, A. C. R.; FARIA, J. M. R. Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers. Journal of Seed Science, Londrina, v. 38 n. 3, p.254-258, July/Sept. 2016.
Resumo: During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.
URI: http://repositorio.ufla.br/jspui/handle/1/31641
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