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Título: | Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification |
Título(s) alternativo(s): | Comportamento de gemas laterais de Hancornia speciosa após criopreservação por encapsulamento-vitrificação |
Palavras-chave: | Mangabeira Recalcitrant species Long-term conservation Dehydration Espécie recalcitrante Conservação a longo prazo Desidratação |
Data do documento: | 2017 |
Editor: | Editora da Universidade Estadual de Maringá (Eduem) |
Citação: | PRUDENTE, D. de O. et al. Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification. Acta Scientiarum. Biological Sciences, Maringá, v. 39, n. 1, p. 87-93, 2017. |
Resumo: | Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. |
URI: | http://repositorio.ufla.br/jspui/handle/1/30153 |
Aparece nas coleções: | DBI - Artigos publicados em periódicos |
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ARTIGO_Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation vitrification.pdf | 1,76 MB | Adobe PDF | Visualizar/Abrir |
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