Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/28562
Title: Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators
Other Titles: Ultraestrutura e citoquímica de calos de pinhão-manso em resposta a balanços entre fitorreguladores
Keywords: Jatropha curcas L.
Embryogenesis
Microscopy
Cytochemistry
Embriogênese
Microscopia
Citoquímica
Issue Date: 2015
Publisher: Editora da Universidade Estadual de Maringá - EDUEM
Citation: Acta Scientiarum. Agronomy
Abstract: This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 µM 2,4-D; (3) 9.04 µM 2,4-D; (4) 4.14 µM PIC; (5) 8.28 µM PIC; (6) 4.52 µM 2,4-D + 2.32 µM KIN; (7) 9.04 µM 2,4-D + 4.64 µM KIN; (8) 4.14 µM PIC + 2.32 µM KIN; (9) 8.28 µM PIC + 4.64 µM KIN; (10) 4.52 µM 2,4-D + 2.22 µM BAP; (11) 9.04 µM 2,4-D + 4.44 µM BAP; (12) 4.14 µM PIC + 2.22 µM BAP and (13) 8.28 µM PIC + 4.44 µM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acetocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 µM of 2,4-D.
URI: http://repositorio.ufla.br/jspui/handle/1/28562
Appears in Collections:DAG - Artigos publicados em periódicos
DFP - Artigos publicados em periódicos



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