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Title: | Fluxo gênico em milho geneticamente modificado com resistência a insetos |
Other Titles: | Gene flow on insect‑resistant genetically modified maize |
Keywords: | Milho Fecundação cruzada Polymerase Chain Reaction - PCR Plantas transgênicas Outcrossing Real‑time PCR Transgenic plants Corn |
Issue Date: | 2012 |
Publisher: | Embrapa Informação Tecnológica Pesquisa Agropecuária Brasileira |
Citation: | NASCIMENTO, V. E. et al. Fluxo gênico em milho geneticamente modificado com resistência a insetos. Pesquisa Agropecuária Brasileira, Brasília, v. 47, n. 6, p. 784-790, jun. 2012. |
Abstract: | The objective of this work was to estimate the gene flow on insect‑resistant transgenic maize in grain production fields. Grain samples were collected in fields containing conventional and transgenic maize in the Brazilian municipalities of: Itumirim, Uberlândia, Paracatu, and Tupaciguara, in the state of Minas Gerais; Itapetininga and Pedrinhas, in the state of São Paulo; and Assaí and Ponta‑Grossa, in the state of Paraná. Seed samples were collected in conventional maize crops starting 5 m away from the transgenic maize source. Ten corn cobs were collected from individual plants per sampling point, with four replicates, totaling 40 corn cobs at each evaluated distance. Gene flow analyses were carried out by real‑time polymerase chain reaction (PCR). On average, 82% of cross‑fertilization occurred within the first 30 m. The highest outcrossing rates were observed in Itapetininga, SP, – at rates above 10% up to a distance of 50 m; however, less than 1% at 100 m distance. The 20-m isolation, with ten border lines, is not sufficient to ensure outcrossing rates lower than 1%. |
URI: | http://repositorio.ufla.br/jspui/handle/1/1487 |
Appears in Collections: | DAG - Artigos publicados em periódicos |
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ARTIGO_Fluxo gênico.pdf | 231,99 kB | Adobe PDF | View/Open |
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