Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/1487
Title: Fluxo gênico em milho geneticamente modificado com resistência a insetos
Other Titles: Gene flow on insect‑resistant genetically modified maize
Keywords: Milho
Fecundação cruzada
Polymerase Chain Reaction - PCR
Plantas transgênicas
Outcrossing
Real‑time PCR
Transgenic plants
Corn
Issue Date: 2012
Publisher: Embrapa Informação Tecnológica Pesquisa Agropecuária Brasileira
Citation: NASCIMENTO, V. E. et al. Fluxo gênico em milho geneticamente modificado com resistência a insetos. Pesquisa Agropecuária Brasileira, Brasília, v. 47, n. 6, p. 784-790, jun. 2012.
Abstract: The objective of this work was to estimate the gene flow on insect‑resistant transgenic maize in grain production fields. Grain samples were collected in fields containing conventional and transgenic maize in the Brazilian municipalities of: Itumirim, Uberlândia, Paracatu, and Tupaciguara, in the state of Minas Gerais; Itapetininga and Pedrinhas, in the state of São Paulo; and Assaí and Ponta‑Grossa, in the state of Paraná. Seed samples were collected in conventional maize crops starting 5 m away from the transgenic maize source. Ten corn cobs were collected from individual plants per sampling point, with four replicates, totaling 40 corn cobs at each evaluated distance. Gene flow analyses were carried out by real‑time polymerase chain reaction (PCR). On average, 82% of cross‑fertilization occurred within the first 30 m. The highest outcrossing rates were observed in Itapetininga, SP, – at rates above 10% up to a distance of 50 m; however, less than 1% at 100 m distance. The 20-m isolation, with ten border lines, is not sufficient to ensure outcrossing rates lower than 1%.
URI: http://repositorio.ufla.br/jspui/handle/1/1487
Appears in Collections:DAG - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_Fluxo gênico.pdf231,99 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons