Please use this identifier to cite or link to this item:
http://repositorio.ufla.br/jspui/handle/1/12579
Title: | Produção de biossurfactantes por leveduras |
Other Titles: | Biosurfactant production by yeast |
Authors: | Dias, Disney Ribeiro Schwan, Rosane Freitas Teixeira, José Antônio Dias, Disney Ribeiro Schwan, Rosane Freitas Batista, Cristina Ferreira Teixeira, José Antônio Gudiña, Eduardo José |
Keywords: | Biossurfactante Agentes antimicrobianos Petróleo – Recuperação por microorganismos Glicolipídeo Fermentação Biosurfactant Antimicrobial agents Petroleum – Microorganism recovery Glycolipid Fermentation |
Issue Date: | 28-Mar-2017 |
Publisher: | Universidade Federal de Lavras |
Citation: | SOUZA, K. S. T. Produção de biossurfactantes por leveduras. 2016. 82 p. Tese (Doutorado em Microbiologia Agrícola)-Universidade Federal de Lavras, Lavras, 2016. |
Abstract: | The main aim of this work was to evaluate the biosufarctant production by five wild yeasts species Yarrowia lipolytica CCMA 0357 (from Amazon soil), Yarrowia lipolytica CCMA 0242 (Kefir), Wickerhamomyces anomalus CCMA 0358 (coffee fermentation), Lindnera saturnus CCMA 0243 (Cerrado soil), and Cryptococcus humicola CCMA 0346 (Cerrado soil), in eight different culture media. Wickerhamomyces anomalus CCMA 0358 cultivated in olive oil presented lowest value of surface tension (32.05 mN/m). A rotatable center composite design (RCCD) was performed for optimization of parameters in order to increase the production of the biosurfactant. The inclusion of yeast extract was 89% significant and the surface tension after optimization was 31.5 mN / m. Batch fermentations were carried out with stirring 500 rpm, and increasing the surface tension of 29.3 mN / m after 24 hours and yield of 2.60 g / L. Antimicrobials and oil recovery tests were performed with the biosurfactant. For the first species of Streptococcus sanguis, Streptococcus oralis and Streptococcus agalactiae, were growth inhibited by 100 % crude biosurfactant, Candida albicans has 78 % inhibition of Staphylococcus epidermitis and 66%. Twenty percent of the oil was recovered using only the supernatant of the fermentation, cell-free, containing the biosurfactant. The biosurfactant studied in this work was characterized as glycolipid, wherein the lipid phase comprises oleic acid. However, the glycidic phase could be identified, indicating that it is a novel compound. These results showed for the first time the production of biosurfactants by Wickerhamomyces anomalus CCMA 0358 which can be used in different biotechnological applications. |
URI: | http://repositorio.ufla.br/jspui/handle/1/12579 |
Appears in Collections: | Microbiologia Agrícola - Doutorado (Teses) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
TESE_Produção de biossurfactantes por leveduras.pdf | 1,54 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.