Please use this identifier to cite or link to this item: http://repositorio.ufla.br/jspui/handle/1/12549
Title: Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies
Other Titles: Identificação de genótipos de tomateiro resistentes a tospovirus em progênies segregantes com marcador molecular
Keywords: Lycopersicon esculentum
Sw-5
Virus resistance
Molecular markers
Resistência a vírus
Marcador molecular
Issue Date: May-2009
Publisher: Escola Superior de Agricultura "Luiz de Queiroz"
Citation: NASCIMENTO, I. R. do N. et al. Marker assisted identification of tospovirus resistant tomato genotypes in segregating progênies. Scientia Agricola, Piracicaba, v. 66, n. 3, p. 298-303, May 2009.
Abstract: The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5+), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.
URI: http://repositorio.ufla.br/jspui/handle/1/12549
Appears in Collections:DFP - Artigos publicados em periódicos



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