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Title: | Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro |
Other Titles: | Microsatelite marker associated with the Ty-1 allele for resistance to Begomovirus in tomato |
Keywords: | Geminiviridae Lycopersicon esculentum Solanum lycopersicum QTL Seleção assistida por marcadores Marker assisted selection Tomateiro - Resistência Begomovirus |
Issue Date: | Apr-2011 |
Publisher: | Embrapa Informação Tecnológica |
Citation: | NOGUEIRA, D. G. et al. Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro. Pesquisa Agropecuária Brasileira, Brasilia, DF, v. 46, n. 4, p. 412-419, abr. 2011. |
Abstract: | ociate a microsatellite marker with the Ty-1 allele that controls resistance to Begomovirus in tomatoes, and to assess the eficiency of this association in the selection of virus-resistant lines. Microsatellite markers SSR-47 and SSR-48 were tested in near isogenic tomato lines (LA-3473, LA-3474, LA-3475) with contrasting genotypes for the Ty-1 allele. The marker SSR-47 detected polymorphism in the lines, and was the only one used in the subsequent research phases. An association between the SSR-47 marker and the Ty-1 locus was detected, and its efficiency for selection of begomovirus-resistant genotypes in tomato was assessed. To confirm the selection efficiency, plants with contrasting banding patterns for the SSR-47 marker were tested for their reaction to Begomovirus. All plants with a single 191-bp band were resistant to Begomovirus, through inoculation test by grafting. Plants with a single 180-bp band were susceptible, and plants with both 191bp and 180 bp bands were resistant. The maximum distance between Ty-1 and the SSR-47 marker was 2.7 cM. This marker was efficient to identify genotypes bearing the Ty-1 allele. Plant responses to infection by Begomovirus inoculated through grafting were consistent with reactions predicted with the use of the SSR-47 marker. |
URI: | http://repositorio.ufla.br/jspui/handle/1/12499 |
Appears in Collections: | DFP - Artigos publicados em periódicos |
Files in This Item:
File | Description | Size | Format | |
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ARTIGO_Marcador microssatélite associado ao alelo Ty-1 de resistência a Begomovirus em tomateiro.pdf | 513,84 kB | Adobe PDF | View/Open |
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