Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/11786
Título: Desenvolvimento de métodos empregando técnicas miniaturizadas de preparo de amostras na determinação residual de anestésico em peixes
Título(s) alternativo(s): Development of methods employing miniaturized sample preparation techniques of determining the residual anesthetic in fish
Autores: Saczk, Adelir Aparecida
Machado, Ana Maria de Resende
Souza, Josefina Aparecida de
Fernandez, Miriany Avelino Moreira
Bernal, Olga Lúcia Mondragón
Palavras-chave: Peixe - Efeito do estresse
Peixe - Resíduos de drogas
Anestésicos
Mentol
Fish - Stress effect
Fish - Drug residues
Anestetics
Menthol
Oreochromis niloticus
Pseudoplatystoma sp.
Data do documento: 19-Set-2016
Editor: Universidade Federal de Lavras
Citação: BOTREL, B. M. C. Desenvolvimento de métodos empregando técnicas miniaturizadas de preparo de amostras na determinação residual de anestésico em peixes. 2016. 220 p. Tese (Doutorado em Agroquímica)-Universidade Federal de Lavras, Lavras, 2016.
Resumo: Fish farming is an activity of great expression in agriculture, and as in most cultures, there are certain managements who are responsible for high stress in fish. Inevitably, this stress directly influences economic performance and welfare of animals, and therefore, the application of drugs is recommended to minimize these effects. However, each drug has a residual period, which badly scaled or not respected, generate waste in fish sold in the food industry. Thus, in order to allow a better standardization of the residual period and fish monitoring, making them safer for consumption, it is essential the development of analytical protocols for the rapid detection of these residues in fish fillet. In this sense, it aimed to the development of analytical methodologies able to determine residual traces of the anesthetic menthol (1R, 2S, 5R) -2-isopropyl-5-metylcyclohexanol fillets of Nile tilapia (Oreochromis niloticus) and catfish (Pseudoplatystoma sp) using the sample preparation techniques: single drop microextraction (SDME), solid phase microextraction (SPME) and dispersive liquid-liquid microextraction (DLLME) and gas chromatography with detection by mass spectrometry (GC-MS). For the development of methods of analysis, the parameters that affect the efficiency of extraction of menthol employed by sample preparation techniques were optimized to obtain the best sensitivity, multivariate analysis. In planning the analyzes it used the rotational central composite design (RCCD), which made it possible to analyze the variables jointly and strategically, resulting in accurate measurement s with less analysis. After determined the best working conditions for the determination of menthol, the methodologies developed were validated and applied to the quantification of residual anesthetic in tilapia fillets and catfish, with considerable precision and accuracy.
URI: http://repositorio.ufla.br/jspui/handle/1/11786
Aparece nas coleções:Agroquímica - Doutorado (Teses)



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