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  2. Instituto de Ciências Naturais - ICN
  3. Departamento de Biologia - DBI
  4. DBI - Artigos publicados em periódicos
Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/39929
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Campo DCValorIdioma
dc.creatorLacerda, Thaís Lourdes Santos-
dc.creatorCardoso, Patrícia Gomes-
dc.creatorAlmeida, LeonardoAugusto de-
dc.creatorCamargo, Ilana Lopes Baratella da Cunha-
dc.creatorAfonso, Daniela Almeida Freitas-
dc.creatorTrant, Cyntia Cardoso-
dc.creatorMacedo, Gilson Costa-
dc.creatorCampos, Eleonora-
dc.creatorCravero, Silvio L.-
dc.creatorSalcedo, Suzana P.-
dc.creatorGorvel, Jean-Pierre-
dc.creatorOliveira, Sérgio Costa-
dc.date.accessioned2020-04-09T20:33:49Z-
dc.date.available2020-04-09T20:33:49Z-
dc.date.issued2010-08-
dc.identifier.citationLACERDA, T. L. S. et al. Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice. Vaccine, [S.l.], v. 28, n. 34, p. 5627-5634, Aug. 2010. DOI: 10.1016/j.vaccine.2010.06.023.pt_BR
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0264410X10008236pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/39929-
dc.description.abstractRough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusΔwbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-α when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. ΔwbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceVaccinept_BR
dc.subjectBrucella abortuspt_BR
dc.subjectRough strainpt_BR
dc.subjectLive vaccinept_BR
dc.titleInactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in micept_BR
dc.typeArtigopt_BR
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