Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/58956
Título: Avaliação da bioatividade de óleo essencial de Eugenia pyriformis sobre Lutzomyia longipalpis e Leishmania infantum
Título(s) alternativo(s): Evaluation of the bioactivity of essential oil from Eugenia pyriformis on Lutzomyia longipalpis and Leishmania infantum
Autores: Barçante, Joziana Muniz de Paiva
Latgé, Samara Graciane da Costa
Latgé, Samara Graciane da Costa
Castro, Joseane Camilla de
Cardoso, Luis
Caicedo Blanco, Yuly Andrea
Palavras-chave: Cafeeiro
Aprendizado de máquina
Potencial hídrico
Análise de dados
Coffee plant
Machine learning
Water potential
Data analysis
Coffee tree
Data do documento: 4-Mar-2024
Editor: Universidade Federal de Lavras
Citação: ALVARENGA, I. M. Avaliação da bioatividade de óleo essencial de Eugenia pyriformis sobre Lutzomyia longipalpis e Leishmania infantum. 2024. 98 p. Tese (Doutorado em Ciências Veterinárias)–Universidade Federal de Lavras, Lavras, 2023.
Resumo: Phlebotomine sandflies are vectors of Leishmania protozoa, posing a significant public health concern. Among the various sandfly species, Lutzomyia longipalpis stands out as the primary transmitter of L. infantum, the etiological agent of Visceral Leishmaniasis (VL), a zoonotic disease with high lethality if not treated early. The four main pillars of control involve health education, early diagnosis and treatment of human cases, control of the canine reservoir, and control of the vector, with the latter three posing significant challenges for health authorities. In this context, the use of botanical compounds has been widely studied, both for application as insecticides and parasiticides. Given the above, the objective of this study was to evaluate the bioactivity of the essential oil from Eugenia pyriformis leaves (OEEp) on vector insects. Additionally, the effect of OEEp on cultivated forms of Leishmania was assessed. OEEp was obtained through steam distillation from moist leaves of Eu. pyriformis collected in Perdões, Minas Gerais. Spectrometry identified β-Caryophyllene (28.21%), Caryophyllene oxide (12.79%), (+)- Spathulenol (6.58%), α-Copaene (5.73%), and β-Gurjunene (5.11%) as major compounds. OEEp was diluted in 4% (v/v) DMSO and used to prepare sugar solutions at concentrations of 2.5 mg/mL, 5 mg/mL, and 7.5 mg/mL, tested in survival, feeding, preference, and attraction assays, all with replicates and controls. To evaluate the leishmanicidal effect of OEEp, promastigotes and amastigotes of Leishmania infantum were used, with Miltefosine as a control. There was no significant difference between OEEp baits and sucrose (control) in survival, attraction, and preference assays. The results indicate that the tested concentrations of OEEp showed no significant differences compared to sucrose control in survival, feeding, attraction, and preference assays. However, when assessing the leishmanicidal potential of OEEp, a significant inhibition of the proliferation of L. infantum promastigotes and amastigotes was observed. Although OEEp did not demonstrate a direct insecticidal effect, its acceptance as a sugar bait by Lu. longipalpis paves the way for integrated strategies that combine bait attractiveness with its leishmanicidal potential.
URI: http://repositorio.ufla.br/jspui/handle/1/58956
Aparece nas coleções:Ciências Veterinárias - Doutorado (Teses)



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