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dc.creatorSilveira, Emanuel Orestes da-
dc.creatorLopes, Ricardo-
dc.creatorRodrigues, Filipe Almendagna-
dc.creatorRaizer, Marcelo Domingues Martins-
dc.creatorDias, Fabio Jacobs-
dc.creatorHarada, Pamela Keiko-
dc.creatorAraújo, Daniele Simões-
dc.creatorPasqual, Moacir-
dc.creatorLuz, José Magno Queiroz-
dc.creatorLopes, Maria Teresa Gomes-
dc.date.accessioned2024-01-11T13:31:32Z-
dc.date.available2024-01-11T13:31:32Z-
dc.date.issued2022-09-09-
dc.identifier.citationSILVEIRA, E. O. da et al. In vitro establishment and multiplication of Echynochloa polystachy. Bioscience Journal, Uberlândia, v. 38, p. 1-12, 2022. DOI: https://doi.org/10.14393/BJ-v38n0a2022-60508.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/58764-
dc.description.abstractThe objective of this work was to carry out the in vitroestablishment of Echynochloa polystachyaaiming at obtaining a micropropagation protocol for works involving the selection of superior genotypes and the cultivation of the species. E. polystachyastems were collected in the municipality of Manaus-AM. Explants were inoculated in test tubes containing Murashige and Skoog (MS) medium. Thirty days after in vitroestablishment, the rate of sprouting and contamination were evaluated. Experiments were also carried out to assess the effects of sucrose and 6-benzylaminopurine (BAP) concentrations on the tillering rate of explants. It was found that during the successive subcultures there was a decrease in internodes and the consequent loss of vigor. There were responses in the multiplication rate at concentrations starting from 45 g L-1sucrose.In addition, BAP and sucrose interfered the development and in vitromultiplication. Sucrose in conjunction with BAP was harmful and shortened internodes. The physiological state of the explants for the species under study was intrinsically linked to the concentrations of sucrose used for the culture medium and the concentrations of BAP. However, the sucrose and BAP concentrations suggested for in vitrocultivation of E. Polystachyamust be adjusted during successive subcultures. Absence of contamination in the in vitroestablishment occurred at concentrations 15, 30 and 60 g L-1sucrose. The combination of 1.5 mg L-1BAP and 30 g L-1sucrose promoted greater induction of sprouts. In addition, the in vitrorooting of E. polystachyawas 45%.pt_BR
dc.languageen_USpt_BR
dc.publisherUniversidade Federal de Uberlândia (UFU)pt_BR
dc.rightsacesso abertopt_BR
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceBioscience Journalpt_BR
dc.subjectCreeping river grasspt_BR
dc.subjectMicropropagationpt_BR
dc.subjectPlant growth regulatorspt_BR
dc.subjectTissue Culturept_BR
dc.titleIn vitro establishment and multiplication of Echynochloa polystachypt_BR
dc.typeArtigopt_BR
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