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Título: Dendrobium nobile in vitro flowering induction
Título(s) alternativo(s): Indução de florescimento in vitro de Dendrobium nobile
Palavras-chave: Flowering
Temperature
Thidiazuron
Orchidaceae
Florescimento
Temperatura
Data do documento: 2023
Editor: Sociedade Brasileira de Floricultura e Plantas Ornamentais
Citação: NADAL, M. C. et al. Dendrobium nobile in vitro flowering induction. Ornamental Horticulture, [S.l.], v. 29, n . 2, p. 135-142, 2023.
Resumo: In vitro flowering is a technique used in genetic improvement that accelerates generations and favors the faster fixation of new traits of agronomic and market interest. The study aimed to establish a protocol for the in vitro flowering of Dendrobium nobile, through the combined temperature and of the growth regulator thidiazuron (TDZ) effects. Experiments was performed in a flask that was kept in a BOD incubator at 18, 21, or 24 °C or in a growth room at 26 °C. The TDZ concentrations were 0.0, 0.5, 1.0, and 2.0 mg L-1. The highest percentage of flowering shoots of 13.7% occurred at a concentration of 2 mg L-1 of TDZ grown at a temperature of 18 °C. Dendrobium nobile in vitro flowering was promoted in plants cultivated in ½ MS supplemented with 30 g L-1 sucrose, 5.5 g L-1 agar, 100 mL L-1 coconut water, pH 6.0, and 2.0 mg L-1 TDZ at a controlled temperature of 18 °C and a photoperiod of 16 h. The in vitro flowering induction protocol of the D. nobile species could be used or improved for future studies.
URI: http://repositorio.ufla.br/jspui/handle/1/58633
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