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dc.creatorSouza, Rafaela Ribeiro de-
dc.creatorPaiva, Patrícia Duarte de Oliveira-
dc.creatorFreitas, Rodrigo Therezan de-
dc.creatorSilva, Diogo Pedrosa Corrêa da-
dc.creatorReis, Michele Valquíria dos-
dc.creatorNery, Fernanda Carlota-
dc.creatorPaiva, Renato-
dc.date.accessioned2023-10-26T20:37:43Z-
dc.date.available2023-10-26T20:37:43Z-
dc.date.issued2023-
dc.identifier.citationSOUZA, R. R. de et al. Cryopreservation of Genipa americana seeds. Revista Ciência Agronômica, [S.l.], v. 54, 2023.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/58478-
dc.description.abstractGenipa americana L. is a forest species of high socioeconomic potential. However, predatory extractivism actions threaten its existence, making it necessary to adopt conservationist practices. G. americana seeds show sensitivity to desiccation and cooling, making it unfeasible for conservation by conventional methods. Thus, cryopreservation is an promising alternative for the long-term conservation of species that produce unorthodox seeds, such as the genipap. In this sense, the objective was to cryopreservation of seeds of G. americana and to evaluate the effects of desiccation and freezing on germination and establishment of seedlings. Initially, seeds were dehydrated in silica gel 0, 16, 18, 20, 22 and 24 h, and then were cryopreserved in liquid nitrogen (LN) (-196 °C) during 24 h. After thawing, the viability and germination were analyzed. Dehydrated and non-cryopreserved seeds were also analyzed. The silica gel desiccation caused a reduction in viability and germination of the seeds of G. americana. The initial seed water content was so high (47%) that storage in LN (+LN) without prior dehydration treatment resulted in seed mortality. It was verified that, the dehydration in silica gel for the minimum time 20 h (corresponding to 14% water content) provides greater freezing tolerance, allowing the successful cryopreservation. Silica gel dehydration followed by immersion in LN was shown to be highly efficient for cryopreservation of seeds of G. americana, besides germinatio after thawing, high survival rates (100%) were obtained, with growth and normal establishment of the seedlings after acclimatization.pt_BR
dc.languageen_USpt_BR
dc.publisherUniversidade Federal do Cearápt_BR
dc.rightsacesso abertopt_BR
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceRevista Ciência Agronômicapt_BR
dc.subjectConservationpt_BR
dc.subjectDehydrationpt_BR
dc.subjectLong-term storagept_BR
dc.subjectRubiaceaept_BR
dc.titleCryopreservation of Genipa americana seedspt_BR
dc.typeArtigopt_BR
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