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Título: | Viability of Campylobacter spp. in frozen and chilled broiler carcasses according to real-time PCR with propidium monoazide pretreatment |
Palavras-chave: | Food safety Campylobacter transmission Propidium monoazide Segurança alimentar Transmissão de campylobacter Monazida de propídio |
Data do documento: | 1-Mai-2018 |
Editor: | Oxford Academic |
Citação: | CASTRO, A. G. S. A. et al. Viability of Campylobacter spp. in frozen and chilled broiler carcasses according to real-time PCR with propidium monoazide pretreatment. Poultry Science, Champaign, v. 97, n. 5, p. 1706-1711, 1 May 2018. |
Resumo: | The aim of this study was to evaluate the viability of Campylobacter spp. in frozen and chilled broiler carcasses using real-time PCR with propidium monoazide (PMA) pretreatment. Sixty broiler carcasses were collected: 30 frozen and 30 chilled. Each carcass was submitted to 2 real-time PCR protocols to detect and quantify Campylobacter spp.: one using pretreatment with PMA, which blocks the amplification of DNA from dead bacteria, and the other without PMA. The results showed that PMA-pretreated carcasses, either frozen or chilled, had a lower positivity rate compared to untreated samples (P < 0.001). Regarding storage temperatures, PMA-pretreated frozen carcasses that tested positive were in a lesser number than chilled carcasses (P < 0.05). However, the quantification of total and live bacteria in PMA-pretreated frozen carcasses that tested positive showed no significant difference compared to chilled carcasses. It was concluded that the real-time PCR with PMA pretreatment was a sensitive method for evaluating the viability of Campylobacter spp. in broiler carcasses. Chilled broiler carcasses would represent greater hazard to public health concerning Campylobacter transmission. |
URI: | https://academic.oup.com/ps/article-abstract/97/5/1706/4876955?redirectedFrom=fulltext http://repositorio.ufla.br/jspui/handle/1/57717 |
Aparece nas coleções: | DMV - Artigos publicados em periódicos |
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