Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/57554
Título: Bone marrow cells of swine: collection and separation
Palavras-chave: Bone marrow
Stem cells
Cell therapy
Medula óssea
Células-tronco
Terapia celular
Data do documento: Jul-2012
Editor: Wiley
Citação: BRANCO, E. et al. Bone marrow cells of swine: collection and separation. Microscopy Research & Technique, New York, v. 75, n. 7, p. 917-920, July 2012.
Resumo: Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy.
URI: https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.22013
http://repositorio.ufla.br/jspui/handle/1/57554
Aparece nas coleções:DMV - Artigos publicados em periódicos

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