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Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Sánchez Viafara, Jesús Alfonso | - |
dc.creator | Vasconcelos, Gisvani Lopes de | - |
dc.creator | Maculan, Renata | - |
dc.creator | Alves, Nadja Gomes | - |
dc.creator | Ferreira, Marcos Brandao Dias | - |
dc.creator | Sudano, Mateus José | - |
dc.creator | Mingoti, Gisele Zoccal | - |
dc.creator | Nunes, Giovana Barros | - |
dc.creator | Lima, Renato Ribeiro de | - |
dc.creator | Drumond, Roberti Martins | - |
dc.creator | Santos, Raphael Nunes dos | - |
dc.creator | Eberlin, Marcos Nogueira | - |
dc.creator | Negrão, Fernanda | - |
dc.creator | Jasmin | - |
dc.creator | Donato, Mariana Aragão M. | - |
dc.creator | Peixoto, Christina A. | - |
dc.creator | Souza, José Camisão de | - |
dc.date.accessioned | 2022-08-19T21:08:06Z | - |
dc.date.available | 2022-08-19T21:08:06Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | SÁNCHEZ VIAFARA, J. A. et al. Peroxisome proliferator-activated receptor delta-PPARδ agonist (L-165041) enhances bovine embryo survival and post vitrification viability. Reproduction, Fertility and Development, Melbourne, v. 34, n. 9, p. 658-668, 2022. DOI: 10.1071/RD21245. | pt_BR |
dc.identifier.uri | https://doi.org/10.1071/RD21245 | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/53339 | - |
dc.description.abstract | The effect of L-165041 (PPARδ-agonist) on decreasing apoptosis and intracellular lipid content was assessed in fresh and vitrified–warmed in vitro-produced bovine embryos. It was hypothesised that the addition of L-165041 to the culture medium enhances development and cryopreservation. Oocytes were allocated to one of two treatments: control-standard culture medium, or L-165041 added to the medium on day 1 with no media change. Ultrastructure, cleavage, and blastocyst rates were evaluated in fresh, and in post-vitrification cultured embryos by optical and electronic microscopy. A subset of fresh embryos were fixed for TUNEL assay and for Sudan-Black-B histochemical staining. Vitrified–warmed embryos were assessed using MALDI-MS technique. Cleavage and blastocyst rates (control 49.4 ± 5.2, L-165041 51.8 ± 4.3) were not influenced by L-165041. The proportion of inner cell mass cells (ICM) was higher in fresh embryos, and the rate of total and ICM apoptosis was lower in L-165041. In warmed-embryos, total and ICM apoptosis was lower in L-165041. The overall hatching rate was higher in L-165041 (66.62 ± 2.83% vs 53.19 ± 2.90%). There was less lipid accumulation in fresh L-165041-embryos. In conclusion, the use of L-165041 is recommended to improve the viability of in vitro-derived bovine embryos. | pt_BR |
dc.language | en_US | pt_BR |
dc.publisher | CSIRO Publishing | pt_BR |
dc.rights | restrictAccess | pt_BR |
dc.source | Reproduction, Fertility and Development | pt_BR |
dc.subject | Bovine embryo - Cryopreservation | pt_BR |
dc.subject | Culture medium | pt_BR |
dc.subject | Embryo apoptosis | pt_BR |
dc.subject | Vitrification | pt_BR |
dc.subject | Embriões bovinos - Criopreservação | pt_BR |
dc.subject | Meio de cultura | pt_BR |
dc.subject | Apoptose do embrião | pt_BR |
dc.subject | Vitrificação | pt_BR |
dc.title | Peroxisome proliferator-activated receptor delta-PPARδ agonist (L-165041) enhances bovine embryo survival and post vitrification viability | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DES - Artigos publicados em periódicos DEX - Artigos publicados em periódicos |
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