Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/50700
Título: Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
Palavras-chave: Microscopy
Toxin
High-performance liquid chromatography
Methods
Data do documento: 2022
Editor: Springer
Citação: BOTELHO, D. M. dos S. et al. Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis. Chemical Papers, [S.l.], v. 76, p. 2567-2572, 2022.
Resumo: Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production.
URI: https://link.springer.com/article/10.1007/s11696-021-01965-5
http://repositorio.ufla.br/jspui/handle/1/50700
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