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Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Silva, Richard Marins da | - |
dc.creator | Guimarães, Valeria Monteze | - |
dc.creator | Veríssimo, Lizzy Ayra Alcântara | - |
dc.creator | Vidigal, Márcia Cristina Teixeira Ribeiro | - |
dc.creator | Minim, Valéria Paula Rodrigues | - |
dc.creator | Minim, Luis Antonio | - |
dc.date.accessioned | 2022-04-07T15:42:00Z | - |
dc.date.available | 2022-04-07T15:42:00Z | - |
dc.date.issued | 2021-10-23 | - |
dc.identifier.citation | SILVA, R. M. da et al. Extraction, purification by cation exchange supermacroporous cryogel and physico-chemical characterization of γ-conglutin from lupin seeds (Lupinus Albus L.). Journal of Separation Science, [S.l.], v. 45, n. 2, p. 401-410, 2021. DOI: 10.1002/jssc.202100675. | pt_BR |
dc.identifier.uri | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202100675 | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/49704 | - |
dc.description.abstract | This study focused on the extraction, purification, and physicochemical characterization of γ-conglutin, a protein present in lupin seeds with properties of reducing blood glucose levels. Total protein was extracted with an alkaline-saline solvent, followed by isoelectric precipitation. Chromatographic purification of the precipitated fraction was performed using a cation exchange supermacroporous cryogel column. Electrophoresis of the eluted fraction from chromatography presented a single band of ∼48 kDa under non-reducing conditions (two bands of ∼30 and ∼17 kDa, under reducing conditions) confirming the success of the purification protocol. Liquid chromatography-tandem mass spectrometry analysis confirmed the identity of the protein as γ-conglutin. The purified γ-conglutin had an isoelectric point of 7.51, β-sheets prevailing as a secondary structure, and denaturation temperature close to 68°C. The outcome of this work showed that γ-conglutin was obtained with a high degree of purity. The proposed purification protocol is simple and can be easily scaled up. | pt_BR |
dc.language | en_US | pt_BR |
dc.publisher | Wiley | pt_BR |
dc.rights | restrictAccess | pt_BR |
dc.source | Journal of Separation Science (JSS) | pt_BR |
dc.subject | Mass spectrometry | pt_BR |
dc.subject | Supermacroporous cryogel | pt_BR |
dc.subject | White lupin | pt_BR |
dc.subject | Y-conglutin purification | pt_BR |
dc.title | Extraction, purification by cation exchange supermacroporous cryogel and physico-chemical characterization of γ-conglutin from lupin seeds (Lupinus Albus L.) | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DCA - Artigos publicados em periódicos |
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