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Campo DCValorIdioma
dc.creatorSilva, Richard Marins da-
dc.creatorGuimarães, Valeria Monteze-
dc.creatorVeríssimo, Lizzy Ayra Alcântara-
dc.creatorVidigal, Márcia Cristina Teixeira Ribeiro-
dc.creatorMinim, Valéria Paula Rodrigues-
dc.creatorMinim, Luis Antonio-
dc.date.accessioned2022-04-07T15:42:00Z-
dc.date.available2022-04-07T15:42:00Z-
dc.date.issued2021-10-23-
dc.identifier.citationSILVA, R. M. da et al. Extraction, purification by cation exchange supermacroporous cryogel and physico-chemical characterization of γ-conglutin from lupin seeds (Lupinus Albus L.). Journal of Separation Science, [S.l.], v. 45, n. 2, p. 401-410, 2021. DOI: 10.1002/jssc.202100675.pt_BR
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202100675pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/49704-
dc.description.abstractThis study focused on the extraction, purification, and physicochemical characterization of γ-conglutin, a protein present in lupin seeds with properties of reducing blood glucose levels. Total protein was extracted with an alkaline-saline solvent, followed by isoelectric precipitation. Chromatographic purification of the precipitated fraction was performed using a cation exchange supermacroporous cryogel column. Electrophoresis of the eluted fraction from chromatography presented a single band of ∼48 kDa under non-reducing conditions (two bands of ∼30 and ∼17 kDa, under reducing conditions) confirming the success of the purification protocol. Liquid chromatography-tandem mass spectrometry analysis confirmed the identity of the protein as γ-conglutin. The purified γ-conglutin had an isoelectric point of 7.51, β-sheets prevailing as a secondary structure, and denaturation temperature close to 68°C. The outcome of this work showed that γ-conglutin was obtained with a high degree of purity. The proposed purification protocol is simple and can be easily scaled up.pt_BR
dc.languageen_USpt_BR
dc.publisherWileypt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceJournal of Separation Science (JSS)pt_BR
dc.subjectMass spectrometrypt_BR
dc.subjectSupermacroporous cryogelpt_BR
dc.subjectWhite lupinpt_BR
dc.subjectY-conglutin purificationpt_BR
dc.titleExtraction, purification by cation exchange supermacroporous cryogel and physico-chemical characterization of γ-conglutin from lupin seeds (Lupinus Albus L.)pt_BR
dc.typeArtigopt_BR
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