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http://repositorio.ufla.br/jspui/handle/1/46911Registro completo de metadados
| Campo DC | Valor | Idioma |
|---|---|---|
| dc.creator | Zorz, Alex Zichner | - |
| dc.creator | Faria, Júlio Cézar Tannure | - |
| dc.creator | Souza, Denys Matheus Santana Costa | - |
| dc.creator | Gonçalves, Douglas Santos | - |
| dc.creator | Oliveira, Leandro Silva de | - |
| dc.creator | Silva, André Luís Lopes da | - |
| dc.creator | Campos, Wellington Ferreira | - |
| dc.creator | Brondani, Gilvano Ebling | - |
| dc.date.accessioned | 2021-08-20T19:04:32Z | - |
| dc.date.available | 2021-08-20T19:04:32Z | - |
| dc.date.issued | 2020-08 | - |
| dc.identifier.citation | ZORZ, A. Z. et al. Microplants production of Eucalyptus cloeziana from indirect organogenesis. Bosque, Valdivia, v. 41, n. 2, p. 113-124, 2020. DOI: 10.4067/S0717-92002020000200113. | pt_BR |
| dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/46911 | - |
| dc.description.abstract | Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana. | pt_BR |
| dc.language | en | pt_BR |
| dc.publisher | Universidad Austral de Chile, Facultad de Ciencias Forestales | pt_BR |
| dc.rights | acesso aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
| dc.source | Bosque (Valdivia) | pt_BR |
| dc.subject | Adventitious rooting | pt_BR |
| dc.subject | Callogenesis | pt_BR |
| dc.subject | Indirect regeneration | pt_BR |
| dc.subject | Adventitious bud | pt_BR |
| dc.subject | Enraizamento adventício | pt_BR |
| dc.subject | Calogênese | pt_BR |
| dc.subject | Organogênese indireta | pt_BR |
| dc.subject | Gema adventícia | pt_BR |
| dc.title | Microplants production of Eucalyptus cloeziana from indirect organogenesis | pt_BR |
| dc.title.alternative | Producción de microplantas de Eucalyptus cloeziana por organogénesis indirecta | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.description.resumo | Resumen: Pocos estudios se han centrado en los métodos de multiplicación in vitro para Eucalyptus cloeziana. En este trabajo se desarrolló un protocolo de organogénesis indirecta para obtener plantas micropropagadas. Se evaluaron las interacciones de los reguladores del crecimiento en tejidos juveniles. Los tejidos del hipocótilo, cotiledón y raíces obtenidos de plántulas germinadas in vitro se sometieron a combinaciones de ácido α-naftaleneacético (ANA), tidiazurón (TDZ) y ácido 2,4-diclorofenoxiacético (2,4-D). Se evaluaron la callogénesis in vitro, la inducción adventicia de yemas, el alargamiento de brotes, y la sobrevivencia y enraizamiento ex vitro. Se observaron estructuras calogénicas en todos los tejidos evaluados. Se observaron características morfogénicas relacionadas con la formación de centros meristemáticos. Los tejidos que presentaron la masa del callo fueron subcultivados en medio de cultivo de regeneración suplementado con 1,0 mg L-1 de 6-bencilaminopurina (BAP), y solo los tejidos del hipocótilo y cotiledón formaron yemas adventicias y brotes alargados. Los tejidos del hipocótilo sometidos a 2,0 mg L-1 de tidiazuron obtuvieron mayor número de brotes alargados. Los dos tejidos (hipocótilo y cotiledón) combinados con TDZ se caracterizaron por sobrevivencia y el enraizamiento adventicio ex vitro de las microestacas. Los resultados confirmaron la eficiencia del cultivo de tejidos para la producción de microplantas por organogénesis indirecta a partir de tejidos del hipocótilo y cotiledón cultivados con TDZ y su implementación puede ser una alternativa para los programas de mejoramiento forestal de E. cloeziana. | pt_BR |
| Aparece nas coleções: | DCF - Artigos publicados em periódicos | |
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| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| ARTIGO_Microplants production of Eucalyptus cloeziana from indirect organogenesis.pdf | 1,62 MB | Adobe PDF | Visualizar/Abrir |
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