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dc.creatorPlescia, Caroline B.-
dc.creatorDavid, Emily A.-
dc.creatorPatra, Dhabaleswar-
dc.creatorSengupta, Ranjan-
dc.creatorAmiar, Souad-
dc.creatorSu, Yuan-
dc.creatorStahelin, Robert V.-
dc.date.accessioned2021-01-05T16:10:43Z-
dc.date.available2021-01-05T16:10:43Z-
dc.date.issued2021-
dc.identifier.citationPLESCIA, C. B et al. SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles. Journal of Biological Chemistry, Bethesda, 100103, 2021. DOI: https://doi.org/10.1074/jbc.RA120.016148.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/45972-
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first discovered in December 2019 in Wuhan, China, and expeditiously spread across the globe causing a global pandemic. Research on SARS-CoV-2, as well as the closely related SARS-CoV-1 and MERS coronaviruses, is restricted to BSL-3 facilities. Such BSL-3 classification makes SARS-CoV-2 research inaccessible to the majority of functioning research laboratories in the United States; this becomes problematic when the collective scientific effort needs to be focused on such in the face of a pandemic. However, a minimal system capable of recapitulating different steps of the viral life cycle without using the virus’ genetic material could increase accessibility. In this work, we assessed the four structural proteins from SARS-CoV-2 for their ability to form virus-like particles (VLPs) from human cells to form a competent system for BSL-2 studies of SARS-CoV-2. Herein, we provide methods and resources of producing, purifying, fluorescently and APEX2-labeling of SARS-CoV-2 VLPs for the evaluation of mechanisms of viral budding and entry as well as assessment of drug inhibitors under BSL-2 conditions. These systems should be useful to those looking to circumvent BSL-3 work with SARS-CoV-2 yet study the mechanisms by which SARS-CoV-2 enters and exits human cells.pt_BR
dc.languageen_USpt_BR
dc.publisherElsevierpt_BR
dc.rightsacesso abertopt_BR
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceJournal of Biological Chemistrypt_BR
dc.subjectCOVID-19pt_BR
dc.subjectCoronaviruspt_BR
dc.subjectSARS-CoV-2pt_BR
dc.subjectElectron microscopypt_BR
dc.subjectVirus buddingpt_BR
dc.subjectMicroscopia eletrônicapt_BR
dc.subjectGeração de víruspt_BR
dc.titleSARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particlespt_BR
dc.typeArtigopt_BR
Aparece nas coleções:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

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