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Título : | Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphologies |
Autor: | Cortese, Mirko Lee, Ji-Young Cerikan, Berati Neufeldt, Christopher J. Oorschot, Viola M. J. Köhrer, Sebastian Hennies, Julian Schieber, Nicole L. Ronchi, Paolo Mizzon, Giulia Brey, Inés Romero Santarella-Mellwig, Rachel Schorb, Martin Boermel, Mandy Mocaer, Karel Beckwith, Marianne S. Templin, Rachel M. Gross, Viktoriia Pape, Constantin Tischer, Christian Frankish, Jamie Horvat, Natalie K. Laketa, Vibor Stanifer, Megan Boulant, Steeve Ruggieri, Alessia Chatel-Chaix, Laurent Schwab, Yannick Schwab, Yannick |
Palavras-chave: | COVID-19 Coronavirus SARS-CoV-2 Viral replication organelles Electron tomography Organelas de replicação viral Tomografia eletrônica |
Publicador: | Elsevier |
Data da publicação: | 2020 |
Referência: | CORTESE, M. et al. Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphologies. Cell Host & Microbe, [S. l.], 2020. DOI: https://doi.org/10.1016/j.chom.2020.11.003. |
Abstract: | Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2 infected human lung epithelial cells. We report 3D electron microscopy reconstructions of whole-cells and subcellular compartments, revealing extensive fragmentation of the Golgi apparatus, alteration of the mitochondrial network and recruitment of peroxisomes to viral replication organelles formed by clusters of double-membrane vesicles (DMVs). These are tethered to the endoplasmic reticulum, providing insights into DMV biogenesis and spatial coordination of SARS-CoV-2 replication. Live cell imaging combined with an infection sensor reveals profound remodeling of cytoskeleton elements. Pharmacological inhibition of their dynamics suppresses SARS-CoV-2 replication. We thus report insights into virus-induced cytopathic effects, and provide alongside a comprehensive publicly available repository of 3D data-sets of SARS-CoV-2 infected cells for download and smooth online visualization. |
URI: | https://www.sciencedirect.com/science/article/pii/S193131282030620X#! http://repositorio.ufla.br/jspui/handle/1/45555 |
Idioma: | en_US |
Aparece nas coleções: | FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19) |
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