Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/45555
Título : Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphologies
Autor: Cortese, Mirko
Lee, Ji-Young
Cerikan, Berati
Neufeldt, Christopher J.
Oorschot, Viola M. J.
Köhrer, Sebastian
Hennies, Julian
Schieber, Nicole L.
Ronchi, Paolo
Mizzon, Giulia
Brey, Inés Romero
Santarella-Mellwig, Rachel
Schorb, Martin
Boermel, Mandy
Mocaer, Karel
Beckwith, Marianne S.
Templin, Rachel M.
Gross, Viktoriia
Pape, Constantin
Tischer, Christian
Frankish, Jamie
Horvat, Natalie K.
Laketa, Vibor
Stanifer, Megan
Boulant, Steeve
Ruggieri, Alessia
Chatel-Chaix, Laurent
Schwab, Yannick
Schwab, Yannick
Palavras-chave: COVID-19
Coronavirus
SARS-CoV-2
Viral replication organelles
Electron tomography
Organelas de replicação viral
Tomografia eletrônica
Publicador: Elsevier
Data da publicação: 2020
Referência: CORTESE, M. et al. Integrative imaging reveals SARS-CoV-2 induced reshaping of subcellular morphologies. Cell Host & Microbe, [S. l.], 2020. DOI: https://doi.org/10.1016/j.chom.2020.11.003.
Abstract: Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2 infected human lung epithelial cells. We report 3D electron microscopy reconstructions of whole-cells and subcellular compartments, revealing extensive fragmentation of the Golgi apparatus, alteration of the mitochondrial network and recruitment of peroxisomes to viral replication organelles formed by clusters of double-membrane vesicles (DMVs). These are tethered to the endoplasmic reticulum, providing insights into DMV biogenesis and spatial coordination of SARS-CoV-2 replication. Live cell imaging combined with an infection sensor reveals profound remodeling of cytoskeleton elements. Pharmacological inhibition of their dynamics suppresses SARS-CoV-2 replication. We thus report insights into virus-induced cytopathic effects, and provide alongside a comprehensive publicly available repository of 3D data-sets of SARS-CoV-2 infected cells for download and smooth online visualization.
URI: https://www.sciencedirect.com/science/article/pii/S193131282030620X#!
http://repositorio.ufla.br/jspui/handle/1/45555
Idioma: en_US
Aparece nas coleções:FCS - Artigos sobre Coronavirus Disease 2019 (COVID-19)

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