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Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Bastos, Sabrina Carvalho | - |
dc.creator | Pimenta, Carlos José | - |
dc.creator | Dias, Disney Ribeiro | - |
dc.creator | Chalfoun, Sara Maria | - |
dc.creator | Angélico, Caroline Lima | - |
dc.creator | Tavares, Lucas Silva | - |
dc.date.accessioned | 2014-10-27T14:05:20Z | - |
dc.date.available | 2014-10-27T14:05:20Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | BASTOS, S. C. et al. Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean. World Journal of Agricultural Sciences, [S.l.], v. 9, n. 2, p. 167-172, 2013. | pt_BR |
dc.identifier.uri | http://www.idosi.org/wjas/wjas9(2)13/9.pdf | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/4487 | - |
dc.description.abstract | To examine tThe optimal conditions of cultivation for Cladosporium cladosporioides to produce and partially purify pectin methylesterase (PME) and polygalacturonase (PG) were examined under laboratory conditions . Activities of PME and PG were evaluated after 10, 15 and 20 days of incubation at 28°C in rice solid medium. Enzyme extraction was carried out by homogenization in buffer solutions at different pH (4, 5 and 6) followed by precipitation with ammonium sulfate [(NH4)2SO4] at 20, 40 and 60% saturation. After 10 days of incubation, the highest activities for PG (105.5 U/mL) and PME (1480 U/mL) in the crude extract was observed. Benzoate buffer (pH 4.0) was the best buffer for enzymes extraction. The final yield of 108.7% for PME and 10.6% for PG and a purification index of 14.2 and 1.4 for PME and PG, respectively, was obtained after saturation with 60%. The ideal conditions for obtained and pre-purification of PME and PG were 10 days of incubation, extraction with benzoate buffer pH 4.0 and precipitation with 60% of (NH4)2SO4. This is the first report on of pectinases pectinolytic enzymes produced by from C. cladosporioides and provides information about growth conditions for production and pre-purification of PME and PG. | pt_BR |
dc.language | en | pt_BR |
dc.publisher | World Science Research Journals (WSRJ) | pt_BR |
dc.rights | restrito | pt_BR |
dc.source | World Journal of Agricultural Sciences (Print) | pt_BR |
dc.subject | Pectinolytic enzymes | pt_BR |
dc.subject | Filamentous fungi | pt_BR |
dc.subject | Pectin | pt_BR |
dc.subject | Coffee fermentation | pt_BR |
dc.title | Pectinases from a new strain of Cladosporium cladosporioides (Fres.) De Vries isolated from coffee bean | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DCA - Artigos publicados em periódicos DNU - Artigos publicados em periódicos |
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