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Campo DCValorIdioma
dc.creatorCardoso, Valdemir Moretto-
dc.creatorCampani Junior, Gilson-
dc.creatorSantos, Maurício P.-
dc.creatorSilva, Gabriel Gonçalves-
dc.creatorPires, Manuella C.-
dc.creatorGonçalves, Viviane M.-
dc.creatorGiordano, Roberto de C.-
dc.creatorSargo, Cíntia R.-
dc.creatorHorta, Antônio C. L.-
dc.creatorZangirolami, Teresa C.-
dc.date.accessioned2020-08-27T16:46:12Z-
dc.date.available2020-08-27T16:46:12Z-
dc.date.issued2020-
dc.identifier.citationCARDOSO, V. M. et al. Cost analysis based on bioreactor cultivation conditions: Production of a soluble recombinant protein using Escherichia coli BL21(DE3). Biotechnology Reports, [S. I.], v. 26, e00441, Jun. 2020. DOI: https://doi.org/10.1016/j.btre.2020.e00441.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/42679-
dc.description.abstractThe impact of cultivation strategy on the cost of recombinant protein production is crucial for defining cost-effective bioreactor operation conditions. This paper presents a methodology to estimate and compare cost impacts related to utilities as well as medium composition, using simple design equations and accessible data. Data from batch bioreactor cultures were used as case study involving the production of pneumococcal surface protein A, a soluble recombinant protein, employing E. coli BL21(DE3). Cultivation strategies and corresponding process costs covered a wide range of operational conditions, including different media, inducers, and temperatures. The core expenses were related to the medium and cooling. When the price of peptone was above the threshold value of US$ 30/kg, defined medium became the best choice. IPTG and temperatures around 32 °C led to shorter cultures and lower PspA4Pro production costs. The procedure offers a simple, accessible theoretical tool to identify cost-effective production strategies using bioreactors.pt_BR
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceBiotechnology Reportspt_BR
dc.subjectBatch culturept_BR
dc.subjectEnergy consumptionpt_BR
dc.subjectProcess economic analysispt_BR
dc.subjectStirred-Tank reactorpt_BR
dc.subjectBioprocess engineeringpt_BR
dc.subjectBiorreatorespt_BR
dc.subjectConsumo de energiapt_BR
dc.subjectAnálise de custopt_BR
dc.subjectReator perfeitamente agitadopt_BR
dc.subjectEngenharia de bioprocessospt_BR
dc.titleCost analysis based on bioreactor cultivation conditions: Production of a soluble recombinant protein using Escherichia coli BL21(DE3)pt_BR
dc.typeArtigopt_BR
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