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dc.creatorSá, Maria Eugênia Lisei de-
dc.creatorLopes, Marcus José Conceição-
dc.creatorCampos, Magnólia de Araújo-
dc.creatorPaiva, Luciano Vilela-
dc.creatorAmorim, Regina Maria Santos de-
dc.creatorBeneventi, Magda Aparecida-
dc.creatorFirmino, Alexandre Augusto Pereira-
dc.creatorSá, Maria Fátima Grossi de-
dc.date.accessioned2020-05-26T15:34:13Z-
dc.date.available2020-05-26T15:34:13Z-
dc.date.issued2012-
dc.identifier.citationSÁ, M. E. L. de et al. Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica. Genetics and Molecular Biology, São Paulo, v. 35, n. 1, p. 272-282, 2012. DOI: 10.1590/S1415-47572012000200008.pt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/41233-
dc.description.abstractSoybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis identified eleven differentially expressed genes encoding putative proteins sharing amino acid sequence similarities by using BlastX: metallothionein, SLAH4 (SLAC1 Homologue 4), SLAH1 (SLAC1 Homologue 1), zinc-finger proteins, AN1-type proteins, auxin-repressed proteins, thioredoxin and nuclear transport factor 2 (NTF-2). Other genes were also found exclusively in nematode stressed soybean roots, such as NAC domain-containing proteins, MADS-box proteins, SOC1 (suppressor of overexpression of constans 1) proteins, thioredoxin-like protein 4-Coumarate-CoA ligase and the transcription factor (TF) MYBZ2. Among the genes identified in non-stressed roots only were Ser/Thr protein kinases, wound-induced basic protein, ethylene-responsive family protein, metallothionein-like protein cysteine proteinase inhibitor (cystatin) and Putative Kunitz trypsin protease inhibitor. An understanding of the roles of these differentially expressed genes will provide insights into the resistance mechanisms and candidate genes involved in soybean-M. javanica interaction and contribute to more effective control of this pathogen.pt_BR
dc.languageen_USpt_BR
dc.publisherSociedade Brasileira de Genética (SBG)pt_BR
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rightsacesso abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.sourceGenetics and Molecular Biologypt_BR
dc.subjectGene expressionpt_BR
dc.subjectRoot knot nematodept_BR
dc.subjectTranscriptomept_BR
dc.titleTranscriptome analysis of resistant soybean roots infected by Meloidogyne javanicapt_BR
dc.typeArtigopt_BR
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