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dc.creatorBustamante, Fernanda de Oliveira-
dc.creatorRocha, Laiane Corsini-
dc.creatorSantos, Natália de Souza-
dc.creatorSilveira, Raphaela Aparecida Duarte-
dc.creatorNunes, Renata de Castro-
dc.creatorMittelmann, Andréa-
dc.creatorTechio, Vânia Helena-
dc.date.accessioned2020-05-01T22:34:19Z-
dc.date.available2020-05-01T22:34:19Z-
dc.date.issued2015-07-
dc.identifier.citationBUSTAMANTE, F. de O. et al. Analysis of nuclear DNA content and chromosome number for screening genotypes and crosses in Annual Ryegrass (Lolium multiflorum Lam.). Australian Journal of Crop Science, [S.l.], v. 9, n. 7, p. 666-670, July 2015.pt_BR
dc.identifier.urihttp://www.cropj.com/bustamante_9_7_2015_666_670.pdfpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/40514-
dc.description.abstractThis study determined the nuclear DNA content and chromosome number for screening genotypes and crosses in L. multiflorum from Brazil. Analyses to estimate nuclear DNA content by flow cytometry were performed in young leaves of four parental genotypes and eight offspring resulting from their crosses. To the chromosome counting, the slides were prepared by flame drying technique and Giemsa staining. The genotypes were classified in the following categories: diploid, triploid, and tetraploid with 14, 21 and 28 chromosomes, respectively. The Average content of DNA in diploids ranged from 5.42pg to 5.85pg. A single triploid plant showed 8.30pg. In tetraploids, the average DNA content ranged from 10.21pg to 10.95pg. Thus, the combination of analyses by DNA quantification and chromosome counting prove to be efficient for screening and diagnosing ploidy level of parents and crosses in Lolium.pt_BR
dc.languageen_USpt_BR
dc.publisherSouthern Cross Publishingpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourceAustralian Journal of Crop Sciencept_BR
dc.subjectAnnual ryegrasspt_BR
dc.subjectCytogeneticspt_BR
dc.subjectFlow cytometrypt_BR
dc.subjectPlant breedingpt_BR
dc.subjectPolyploidypt_BR
dc.titleAnalysis of nuclear DNA content and chromosome number for screening genotypes and crosses in Annual Ryegrass (Lolium multiflorum Lam.)pt_BR
dc.typeArtigopt_BR
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