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dc.creatorCampos, J. M. S.-
dc.creatorDavide, L. C.-
dc.creatorSalgado, C. C.-
dc.creatorSantos, F. C.-
dc.creatorCosta, P. N.-
dc.creatorSilva, P. S.-
dc.creatorAlves, C. C. S.-
dc.creatorViccini, L. F.-
dc.creatorPereira, A. V.-
dc.date.accessioned2020-02-12T14:36:05Z-
dc.date.available2020-02-12T14:36:05Z-
dc.date.issued2009-02-
dc.identifier.citationCAMPOS, J. M. S. et al. In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum. Plant Breeding, [S. l.], v. 128, n. 1, p. 101-104, Feb. 2009.pt_BR
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0523.2008.01546.xpt_BR
dc.identifier.urihttp://repositorio.ufla.br/jspui/handle/1/39004-
dc.description.abstractThis study describes an efficient in vitro method using colchicine‐treated triploid seedlings of Napiergrass and Pearl millet hybrids to produce hexaploid hybrids and their subsequent identification with flow cytometry. It also describes chromosome count and stomatal morphology and their use to ploidy analysis. Four‐hundred and eighty triploid seeds, representing 12 different hybrids were sterilized and transferred to MS media to induce chromosome doubling. Surviving plants were analysed by flow cytometry. From six triploid (control) and hexaploid plants, the stomata sizes and frequency were analysed. Chromosome count was performed only in the plants identified as hexaploid. Seventeen plants were identified as hexaploid by flow cytometry analysis. Further confirmation of the hexaploid condition was performed with stomatal morphology (stomatal frequency reduction and stomatal length increase) and chromosome count (2n = 6x = 42). Chromosome doubling has numerous applications in Pennisetum breeding. It can be used to restore the fertility of interspecific hybrids and to improve seed size.pt_BR
dc.languageen_USpt_BR
dc.publisherBlackwell Verlagpt_BR
dc.rightsrestrictAccesspt_BR
dc.sourcePlant Breedingpt_BR
dc.subjectChromosome doublingpt_BR
dc.subjectColchicinept_BR
dc.subjectPennisetum hybridspt_BR
dc.subjectFlow cytometrypt_BR
dc.subjectDuplicação cromossômicapt_BR
dc.subjectColchicinapt_BR
dc.subjectCitometria de fluxopt_BR
dc.titleIn vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucumpt_BR
dc.typeArtigopt_BR
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