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Título: | Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings |
Título(s) alternativo(s): | Solução desinfestante no crescimento in vitro de plântulas de Byrsonima intermedia |
Palavras-chave: | Micropropagation Native plant Tissue culture pH NaOCl Sodium hypochlorite Micropropagação Planta nativa Cultura de tecidos Hipoclorito de sódio |
Data do documento: | Abr-2015 |
Editor: | Universidade Federal de Santa Maria (UFSM) |
Citação: | SILVA, L. C. et al. Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings. Ciência Rural, Santa Maria, v. 45, n. 4, p. 674-679, Apr. 2015. DOI: 10.1590/0103-8478cr20120078. |
Resumo: | Byrsonima intermedia A. Juss., is a medicinal and fruit plant of the Cerrado in which the conventional propagation is difficult due to the presence of extremely lignified endocarps. Although sodium hypochlorite (NaOCl) is widely used in the surface decontamination, there are few reports of its effects on explant growth. The aim of this work was to study the effect of different pH and exposure periods of B. intermedia seeds to a NaOCl solution. Seeds were subjected to different exposure periods (1, 5 and 10 minutes) to a NaOCl solution at different pH (5, 7, 10 and 12) and after treatment with NaOCl, embryos were inoculated in a WPM medium with 50% concentration of salts without sucrose, 0.5% agar and pH 5.8 and after 75 days of culture the growth of seedlings was evaluated. The use of NaOCl is effective in the decontamination of B. intermedia seeds, independent of pH variation and exposure periods and the parameters such as percentage of normal seedlings, shoot length, and number of leaves are positively affected by the use of NaOCl solution at pH 8.5-8.9 and by increasing the exposure period, however, the number of roots is affected only by increasing the exposure period in the NaOCl solution. |
URI: | http://repositorio.ufla.br/jspui/handle/1/38999 |
Aparece nas coleções: | DBI - Artigos publicados em periódicos |
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ARTIGO_Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings.pdf | 595,78 kB | Adobe PDF | Visualizar/Abrir |
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