Use este identificador para citar ou linkar para este item: http://repositorio.ufla.br/jspui/handle/1/37863
Título: Genetic variability among cassava accessions based on SSR markers
Título(s) alternativo(s): Variabilidade genética entre acessos de mandioca com base em marcadores SSR
Palavras-chave: Manihot esculenta
Microssatélites
Microsatellites
Data do documento: 2011
Editor: Crop Breeding and Applied Biotechnology
Citação: RIBEIRO, M. de N. O.; CARVALHO, S. P. de; SANTOS, J. B. dos; ANTONIO, R. P. Genetic variability among cassava accessions based on SSR markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 11, n. 3, p. 263-269, 2011.
Resumo: The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
URI: http://repositorio.ufla.br/jspui/handle/1/37863
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