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Título: | Aspectos fisiológicos e bioquímicos da germinação de sementes de alface em diferentes temperaturas |
Título(s) alternativo(s): | Physiological and biochemical aspects of lettuce seed germination at different temperatures |
Palavras-chave: | Lactuca sativa Endo-β-mananase Proteínas tolerantes ao calor Termoinibição Heat-tolerant proteins Thermoinhibition |
Data do documento: | Abr-2014 |
Editor: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Secretaria de Pesquisa e Desenvolvimento (SPD) |
Citação: | CATÃO, H. C. R. M. et al. Physiological and biochemical aspects of lettuce seed germination at different temperatures. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 4, p. 316-322, abr. 2014. DOI: 10.1590/S0100-204X2014000400010. |
Resumo: | The objective of this work was to evaluate the electrophoretic patterns of heat-tolerant proteins and the activity of the endo-β-mannanase enzyme during lettuce seed germination, at high temperature. Seeds of eight lettuce cultivars were subjected to germination, first count, and emergence tests at two temperatures, 20 and 35ºC. The index of germination speed (IVG) and the emergence rate index (IVE) were calculated. The expression of heat-tolerant proteins and of the endo-β-mannanase enzyme was also evaluated for all treatments. The experimental design was completely randomized, in a 2x8 factorial arrangement, with two temperatures and eight cultivars. The highest germination is observed at 35ºC in the Everglades cultivar, considered thermotolerant. The patterns of heat-tolerant proteins in lettuce seeds present specific bands in the Everglades cultivar, at 35ºC. The activity of the endo-β-mannanase enzyme is higher in the Everglades cultivar, at this temperature. This cultivar has potential for use in lettuce breeding programs aiming for tolerance to high temperatures during germination. |
URI: | http://repositorio.ufla.br/jspui/handle/1/37430 |
Aparece nas coleções: | DAG - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_Aspectos fisiológicos e bioquímicos da germinação de sementes de alface em diferentes temperaturas.pdf | 746,48 kB | Adobe PDF | Visualizar/Abrir |
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