Use este identificador para citar ou linkar para este item:
http://repositorio.ufla.br/jspui/handle/1/36381
Registro completo de metadados
Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Ascari, I. J. | - |
dc.creator | Alves, N. G. | - |
dc.creator | Jasmin, J. | - |
dc.creator | Lima, R. R. | - |
dc.creator | Quintão, C. C. R. | - |
dc.creator | Oberlender, G. | - |
dc.creator | Moraes, E. A. | - |
dc.creator | Camargo, L. S. A. | - |
dc.date.accessioned | 2019-08-21T17:20:43Z | - |
dc.date.available | 2019-08-21T17:20:43Z | - |
dc.date.issued | 2017-07 | - |
dc.identifier.citation | ASCARI, I. J. et al. Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. Domestic Animal Endocrinology, Stoneham, v. 60, p. 50-60, July 2017. | pt_BR |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0739724017300589?via%3Dihub#! | pt_BR |
dc.identifier.uri | http://repositorio.ufla.br/jspui/handle/1/36381 | - |
dc.description.abstract | This study was performed to investigate the effects of insulin-like growth factor-I (IGF-I) addition to in vitro maturation (IVM) medium on apoptosis, mitochondrial membrane potential, ROS production, and developmental competence of bovine oocytes subjected to heat shock. Two temperatures (conventional: 24 h at 38.5°C, or heat shock: 12 h at 41°C followed by 12 h at 38.5°C) and 3 IGF-I concentrations (0, 25, and 100 ng/mL) were tested during IVM. The oocytes were then fertilized in vitro, and the presumptive zygotes were cultured until reaching the blastocyst stage. There was no interaction between temperature and IGF-I concentration for any variable evaluated (P > 0.05). The addition of IGF-I did not alter the proportion of nuclear maturation, TUNEL-positive oocytes and caspase-3 activity, or blastocyst proportion on Days 7 and 8 post-fertilization. Furthermore, the total number of cells and the number of cells in the inner cell mass (ICM) in the blastocyst were not altered (P > 0.05). However, IGF-I increased (P < 0.05) the mitochondrial membrane potential and the production of ROS in oocytes and decreased (P < 0.05) the proportion of apoptotic cells in the ICM in blastocysts. Heat shock increased (P < 0.05) the proportion of TUNEL-positive oocytes and ROS production and reduced (P < 0.05) the mitochondrial membrane potential. Moreover, heat shock increased (P < 0.05) the apoptosis proportion in the ICM cells. In conclusion, supplementing IVM medium with IGF-I may increase the mitochondrial membrane potential and ROS production in oocytes and decrease apoptosis in the ICM in blastocysts. Heat shock for 12 h compromised oocyte developmental competence and increased apoptosis within the ICM cells of the blastocysts. | pt_BR |
dc.language | en_US | pt_BR |
dc.publisher | Elsevier | pt_BR |
dc.rights | restrictAccess | pt_BR |
dc.source | Domestic Animal Endocrinology | pt_BR |
dc.subject | Thermoprotection | pt_BR |
dc.subject | Heat stress | pt_BR |
dc.subject | DNA fragmentation | pt_BR |
dc.subject | Mitochondrial membrane potential | pt_BR |
dc.subject | ROS production | pt_BR |
dc.subject | Termoproteção | pt_BR |
dc.subject | Estresse por calor | pt_BR |
dc.subject | Fragmentação de DNA | pt_BR |
dc.subject | Potencial de membrana mitocondrial | pt_BR |
dc.subject | Produção de ROS | pt_BR |
dc.title | Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence | pt_BR |
dc.type | Artigo | pt_BR |
Aparece nas coleções: | DES - Artigos publicados em periódicos |
Arquivos associados a este item:
Não existem arquivos associados a este item.
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.